A complete set of repeat-depleted, PCR-amplifiable, human chromosome-specific painting probes

被引：21

作者：

Bolzer, A ^{[1
]}

Craig, JM ^{[1
]}

Cremer, T ^{[1
]}

Speicher, MR ^{[1
]}

机构：

[1] Univ Munich, Inst Anthropol & Human Genet, D-80336 Munich, Germany

来源：

CYTOGENETICS AND CELL GENETICS | 1999年 / 84卷 / 3-4期

关键词：

D O I：

10.1159/000015266

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We describe the generation of a complete set of human chromosome-specific painting probes depleted in repetitive sequences. These probes yield highly specific signals when hybridized without the addition of a blocking agent, such as Cot-1 DNA, and without probe preannealing prior to hybridization. Fluorescent intensities and signal-to-background ratios for these probes are comparable to those of untreated probes hybridized with Cot-1 DNA. We demonstrate the suitability of these probes for applications with very complex probe sets, such as multiplex-FISH.

引用

页码：233 / 240

页数：8

共 27 条

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