Sensitivity of histamine H-3 receptor agonist-stimulated [S-35]GTP gamma[S] binding to pertussis toxin

被引：80

作者：

Clark, EA ^{[1
]}

Hill, SJ ^{[1
]}

机构：

[1] QUEENS MED CTR,SCH MED,DEPT PHYSIOL & PHARMACOL,NOTTINGHAM NG7 2UH,ENGLAND

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY | 1996年 / 296卷 / 02期

基金：

英国医学研究理事会;

关键词：

S-35]GTP gamma[S] binding; histamine H-3 receptor; G-protein; pertussis toxin;

D O I：

10.1016/0014-2999(95)00800-4

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The effect of histamine H-3 receptor-selective ligands on [S-35]guanosine 5'-o-(gamma-thio)triphosphate ([S-35]GTP gamma[S]) binding has been examined in rat cerebral cortical membranes. R(alpha)-Methylhistamine and N-alpha-methylhistamine produced a concentration-dependent stimulation of [S-35]GTP gamma[S] binding which was attenuated in the presence of the selective histamine H-3 receptor antagonist thioperamide. In addition, treatment of brain membranes with pertussis toxin abolished the histamine H-3 receptor agonist stimulated binding of [S-35]GTP gamma[S]. These results provide the first evidence that histamine H-3 receptors couple directly to a G(i)/G(o) protein in mammalian brain.

引用

页码：223 / 225

页数：3

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