Real-time multiplex PCR assay for detection of Brucella spp., B-abortus, and B-melitensis

被引：179

作者：

Probert, WS ^{[1
]}

Schrader, MN ^{[1
]}

Khuong, NY ^{[1
]}

Bystrom, SL ^{[1
]}

Graves, MH ^{[1
]}

机构：

[1] Calif Dept Hlth Serv, Div Communicable Dis Control, Microbial Dis Lab, Richmond, CA 94804 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 03期

关键词：

D O I：

10.1128/JCM.42.3.1290-1293.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The identification of Brucella can be a time-consuming and labor-intensive process that places personnel at risk for laboratory-acquired infection. Here, we describe a real-time PCR assay for confirmation of presumptive Brucella isolates. The assay was designed in a multiplex format that will allow the rapid identification of Brucella spp., B. abortus, and B. melitensis in a single test.

引用

页码：1290 / 1293

页数：4

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