Formation and characterization of planar lipid bilayer membranes from synthetic phytanyl-chained glycolipids

被引:60
作者
Baba, T
Toshima, Y
Minamikawa, H
Hato, M
Suzuki, K
Kamo, N
机构
[1] Natl Inst Mat & Chem Res, Surface Engn Lab, Tsukuba, Ibaraki 3058565, Japan
[2] Natl Inst Sericultural & Entomol Sci, Tsukuba, Ibaraki 3058634, Japan
[3] Daiichi Pharmaceut Co Ltd, Tokyo R&D Ctr, Edogawa Ku, Tokyo 1348630, Japan
[4] Hokkaido Univ, Fac Pharmaceut Sci, Kita Ku, Sapporo, Hokkaido 0600812, Japan
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1999年 / 1421卷 / 01期
关键词
planar lipid bilayer membrane; phytanyl-chained glycolipid; membrane conductance; membrane stability; lipophilic ion; ion channel incorporation;
D O I
10.1016/S0005-2736(99)00114-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The formability, current-voltage characteristics and stability of the planar lipid bilayer membranes from the synthetic phytanyl-chained glycolipids, 1,3-di-O-phytanyl-2-O-(beta-glycosyl)glycerols (Glc(Phyt)(2), Mal(N)(Phyt)(2)) were studied. The single bilayer membranes were successfully formed from the glycolipid bearing a maltotriosyl group (Mal(3)(Phyt)(2)) by the folding method among the synthetic glycolipids examined. The membrane conductance of Mal(3)(Phyt)(2) bilayers in 100 mM KCl solution was significantly lower than that of natural phospholipid, soybean phospholipids (SBPL) bilayers, and comparable to that of 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC) bilayers. From the permeation measurements of lipophilic ions through Mal(3)(Phyt)(2) and DPhPC bilayers, it could be presumed that the carbonyl groups in glycerol backbone of the lipid molecule are not necessarily required for the total dipole potential barrier against cations in Mal(3)(Phyt)(2) bilayer. The stability of Mal(3)(Phyt)(2) bilayers against long-term standing and external electric field change was rather high, compared with SBPL bilayers, Furthermore, a preliminary experiment over the functional incorporation of membrane proteins was demonstrated employing the channel proteins derived from octopus retina microvilli vesicles. The channel proteins were functionally incorporated into Mal(3)(Phyt)(2) bilayers in the presence of a negatively charged glycolipid. From these observations, synthetic phytanyl-chained glycolipid bilayers are promising materials for reconstitution and transport studies of membrane proteins. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:91 / 102
页数:12
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