Antibody-free method for protein detection on blots using enzyme fragment complementation

被引：5

作者：

Horecka, J ^{[1
]}

Charter, NW ^{[1
]}

Bosano, BL ^{[1
]}

Fung, P ^{[1
]}

Kobel, P ^{[1
]}

Peng, K ^{[1
]}

Eglen, RM ^{[1
]}

机构：

[1] DiscoveRx Corp, Fremont, CA 94538 USA

来源：

BIOTECHNIQUES | 2006年 / 40卷 / 03期

关键词：

D O I：

10.2144/000112119

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：381 / 383

页数：3

共 9 条

[1] Enzyme fragment complementation: A flexible high throughput screening assay technology [J].

Eglen, RM .

ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES, 2002, 1 (01) :97-104

[2] NFAT: ubiquitous regulator of cell differentiation and adaptation [J].

Horsley, V ;

Pavlath, GK .

JOURNAL OF CELL BIOLOGY, 2002, 156 (05) :771-774

[3] Protein blotting: a review [J].

Kurien, BT ;

Scofield, RH .

JOURNAL OF IMMUNOLOGICAL METHODS, 2003, 274 (1-2) :1-15

[4] NFAT proteins: Key regulators of T-cell development and function [J].

Macian, F .

NATURE REVIEWS IMMUNOLOGY, 2005, 5 (06) :472-484

[5]

McIlhinney R A Jeffrey, 2004, Methods Mol Biol, V259, P81

[6] β galactosidase enzyme fragment complementation as a high-throughput screening protease technology [J].

Naqvi, T ;

Lim, A ;

Rouhani, R ;

Singh, R ;

Eglen, RM .

JOURNAL OF BIOMOLECULAR SCREENING, 2004, 9 (05) :398-408

[7] Detection technologies in proteome analysis [J].

Patton, WF .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 771 (1-2) :3-31

[8] A high-throughput, nonisotopic, competitive binding assay for kinases using nonselective inhibitor probes (ED-NSIP™) [J].

Vainshtein, I ;

Silveria, S ;

Kaul, P ;

Rouhani, R ;

Eglen, RM ;

Wang, J .

JOURNAL OF BIOMOLECULAR SCREENING, 2002, 7 (06) :507-514

[9] Homogeneous assays for cellular protein degradation using β-galactosidase complementation:: NF-κB/IκB pathway signaling [J].

Zhao, XN ;

Vainshtein, I ;

Gellibolian, R ;

Shu, YL ;

Dotimas, H ;

Wang, XM ;

Fung, P ;

Horecka, J ;

Bosano, BL ;

Eglen, RM .

ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES, 2003, 1 (06) :823-833

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