Myoglobin immobilized on Fe3O4@SiO2 magnetic nanoparticles:: Direct electron transfer, enhanced thermostability and electroactivity

被引：23

作者：

Xu, Qin ^{[1
]}

Bian, Xiao-Jun ^{[1
]}

Li, Liang-Liang ^{[1
]}

Hu, Xiao-Ya ^{[1
]}

Sun, Min ^{[1
]}

Da Chen ^{[1
]}

Wang, Yang ^{[1
]}

机构：

[1] Yangzhou Univ, Coll Chem & Chem Engn, Yangzhou 225002, Peoples R China

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2008年 / 10卷 / 07期

基金：

中国国家自然科学基金;

关键词：

magnetic nanoparticles; myoglobin; immobilization; thermostability;

D O I：

10.1016/j.elecom.2007.12.002

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

Myoglobin (Mb) has been successfully immobilized in alternation with oppositely charged poly(dimethyldiallylammonium chloride) via the sequential layering approach on the biocompatible Fe3O4@SiO2 nanoparticles. The bound Mb could be easily separated by an external magnetic field and used as less costly, more stable, and reusable alternatives to the soluble ones. Direct electron transfer between the immobilized Mb and the electrode was observed. Moreover, the immobilized Mb provided remarkable thermostability up to 70 degrees C and high electroactivity with the apparent Michaelis-Menten constant (k(M)) of 45 mu M. (c) 2007 Published by Elsevier B.V.

引用

页码：995 / 999

页数：5

共 18 条

[1]

DAI Z, 2004, ANAL BIOCHEM, V332, P332

[2] Fuzzy nanoassemblies: Toward layered polymeric multicomposites [J].

Decher, G .

SCIENCE, 1997, 277 (5330) :1232-1237

[3] HAMMETT RHO-SIGMA CORRELATION FOR REACTIONS OF HORSERADISH-PEROXIDASE COMPOUND-II WITH PHENOLS [J].

DUNFORD, HB ;

ADENIRAN, AJ .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1986, 251 (02) :536-542

[4]

ENOKI Y, 1986, BIOCHIM BIOPHYS ACTA, V789, P331

[5]

F Allen J Bard L.R., 2001, Electrochemical Methods: Fundamentals and Applications

[6] A SPECTROPHOTOMETRIC STUDY OF IONIZATIONS IN METHAEMOGLOBIN [J].

GEORGE, P ;

HANANIA, G .

BIOCHEMICAL JOURNAL, 1953, 55 (02) :236-243

[7] Surface-linked molecular monolayers of an engineered myoglobin: Structure, stability, and function [J].

Jiang, M ;

Nolting, B ;

Stayton, PS ;

Sligar, SG .

LANGMUIR, 1996, 12 (05) :1278-1283

[8]

LI H, 2005, J PHYS CHEM-US, V109, P10464

[9] Preparation of nanoporous polyelectrolyte multilayer films via nanoparticle templating [J].

Li, Qi ;

Quinn, John F. ;

Wang, Yajun ;

Caruso, Frank .

CHEMISTRY OF MATERIALS, 2006, 18 (23) :5480-5485

[10] Effects of the concentration of tetramethylammonium hydroxide peptizer on the synthesis of Fe3O4/SiO2 core/shell nanoparticles [J].

Lu, ZY ;

Wang, G ;

Zhuang, JQ ;

Yang, WS .

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2006, 278 (1-3) :140-143

← 1 2 →