The role of ultraviolet radiation, photosensitizers, reactive oxygen species and ester groups in mechanisms of methane formation from pectin

被引:107
作者
Messenger, David J. [1 ,2 ]
McLeod, Andy R. [2 ]
Fry, Stephen C. [1 ]
机构
[1] Univ Edinburgh, Sch Biol Sci, Inst Mol Plant Sci, Edinburgh, Midlothian, Scotland
[2] Univ Edinburgh, Sch Geosci, Inst Atmospher & Environm Sci, Edinburgh, Midlothian, Scotland
基金
英国自然环境研究理事会; 英国生物技术与生命科学研究理事会;
关键词
acetyl esters; CH4; homogalacturonan methyl ester; hydroxyl radical; pectic polysaccharides; plant cell walls; ROS; singlet oxygen; UV; PRIMARY-CELL WALL; HYDROXYL-RADICAL PRODUCTION; ATMOSPHERIC METHANE; TERRESTRIAL PLANTS; OXIDATIVE BURST; SINGLET OXYGEN; IN-VITRO; EMISSION; POLYSACCHARIDES; ASCORBATE;
D O I
10.1111/j.1365-3040.2008.01892.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Ultraviolet (UV) radiation has recently been demonstrated to drive an aerobic production of methane (CH4) from plant tissues and pectins, as do agents that generate reactive oxygen species (ROS) in vivo independently of UV. As the major building-blocks of pectin do not absorb solar UV found at the earth's surface (i.e. > 280 nm), we explored the hypothesis that UV radiation affects pectin indirectly via generation of ROS which themselves release CH4 from pectin. Decreasing the UV absorbance of commercial pectin by ethanol washing diminished UV-dependent CH4 production, and this was restored by the addition of the UV photosensitizer tryptophan. Certain ROS scavengers [mannitol, a hydroxyl radical ((OH)-O-center dot) scavenger; 1,4-diazabicyclo[2.2.2] octane; and iodide] strongly inhibited UV-induced CH4 production from dry pectin. Furthermore, pectin solutions emitted CH4 in darkness upon the addition of (OH)-O-center dot, but not superoxide or H2O2. Model carbohydrates reacted similarly if they possessed -CH3 groups [e.g. methyl esters or (more weakly) acetyl esters but not rhamnose]. We conclude that UV evokes CH4 production from pectic methyl groups by interacting with UV photosensitizers to generate (OH)-O-center dot. We suggest that diverse processes generating (OH)-O-center dot could contribute to CH4 emissions independently of UV irradiation, and that environmental stresses and constitutive physiological processes generating ROS require careful evaluation in studies of CH4 formation from foliage.
引用
收藏
页码:1 / 9
页数:9
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