Recent developments in single-cell analysis

被引：61

作者：

Lu, X ^{[1
]}

Huang, WH ^{[1
]}

Wang, ZL ^{[1
]}

Cheng, HK ^{[1
]}

机构：

[1] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2004年 / 510卷 / 02期

基金：

中国国家自然科学基金;

关键词：

single-cell analysis; capillary electrophoresis; imaging analysis; temporal and spatial monitoring;

D O I：

10.1016/j.aca.2004.01.014

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Cells play a significant role in life activities such as metabolism and signal transduction and so on. The development of cellular research puts forward a severe challenge to analyst, and the study of single-cell has already become the focus of the frontiers in analytical chemistry. Combining capillary electrophoresis (CE) with detection methods such as laser-induced fluorescence (LIF) and electrochemical detection (ED), and mass spectrometry (MS) have particular description for single-cell analysis. Due to extensive research on objects and complex characteristic in the single-cell, scientists have developed more methods and technologies for application in single-cell analysis. Here we reviewed the current approach of technologies of single-cell analysis in manipulation, injection and lysis of single-cell, capillary electrophoresis, imaging analysis, temporal and spatial monitoring on dynamics of single-cells, and the application. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：127 / 138

页数：12

共 112 条

[1] Distribution of zeptornole-abundant doxorubicin metabolites in subcellular fractions by capillary electrophoresis with laser-induced fluorescence detection [J].