Clonal relationships among Escherichia coli serogroup O6 isolates based on RAPD

被引：15

作者：

Pacheco, ABF ^{[1
]}

Guth, BEC ^{[1
]}

Soares, KCC ^{[1
]}

deAlmeida, DF ^{[1
]}

Ferreira, LCS ^{[1
]}

机构：

[1] UNIV FED SAO PAULO,DEPT MICROBIOL IMMUNOL & PARASITOL,ESCOLA PAULISTA MED,BR-04023062 SAO PAULO,BRAZIL

来源：

FEMS MICROBIOLOGY LETTERS | 1997年 / 148卷 / 02期

基金：

巴西圣保罗研究基金会;

关键词：

Escherichia coli; RAPD typing; clonal grouping; serotype;

D O I：

10.1016/S0378-1097(97)00045-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The genetic diversity in a group of Escherichia coli strains belonging to serogroup O6 but expressing different H antigens was investigated by random amplification of polymorphic DNA (RAPD). Isolates of serotypes H16, H1, H31, and non-motile (NM) strains were typed using a set of 3 primers with different G+C contents. The amplified band arrays allowed the identification of 3 main clonal clusters corresponding to each O:H serotype analyzed. Based on their RAPD profiles NEVI strains could be assigned to either H1 or H31 serotypes. The results indicate that the flagellar antigen and the RAPD fingerprint represent reliable clonal markers in this E. coli group.

引用

页码：255 / 260

页数：6

共 18 条

[1]

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[2] GENETIC DIVERSITY IN RELATION TO SEROTYPE IN ESCHERICHIA-COLI [J].