Bicuculline-resistant, Cl- dependent GABA response in the rat spinal dorsal horn

被引:10
作者
Park, JS
Higashi, H
Nagata, K
Yoshimura, M
机构
[1] Saga Med Sch, Dept Physiol, Saga 849, Japan
[2] Kurume Univ, Sch Med, Dept Physiol, Kurume, Fukuoka 830, Japan
[3] Kurume Univ, Sch Med, Dept Orthoped, Kurume, Fukuoka 830, Japan
关键词
GABA(C); bicuculline resistant; CACA; substantia gelatinosa; spinal cord; GABA receptor subunit;
D O I
10.1016/S0168-0102(99)00016-4
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Receptors for gamma-aminobutyric acid (GABA), the main inhibitory neurotransmitter in the mammalian central nervous system (CNS), have been divided into three subtypes. GABA(A) receptor is a ligand-gated chloride channel that is competitively antagonized by bicuculline, whereas GABA(B) receptor regulate Ca2+ or K+ channels through G proteins. Recently, GABA(C) receptor has been identified in mammalian and fish retina. Unlike GABA(A) receptors, the GABA(C) receptor is a bicuculline-resistant chloride channel that is selectively activated by cis-4-aminocrotonic acid (CACA), and antagonized by imidazole-4-acetic acid (I4AA) and to some extent by picrotoxin. We report here that bicuculline-resistant GABA responses mediated by chloride channels are also expressed in substantia gelatinosa (SG) neurons in the dorsal horn, which receive predominantly nociceptive inputs from periphery. The GABA responses are, however, not mimicked by CACA nor affected by I4AA, but abolished by picrotoxin. Moreover, these responses are modulated by benzodiazepines (flunitrazepam) and barbiturates (thiopental), although GABA(C) responses are not affected. Thus, the pharmacological characteristics of the GABA responses observed in SG neurons are distinct from those responses mediated by the known GABA receptors. These differences may reflect the presence of receptor subunits unique to SG neurons. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:261 / 268
页数:8
相关论文
共 44 条
[1]   EXCITATORY AND INHIBITORY-ACTION OF GABA ON SYNAPTIC TRANSMISSION IN SLICES OF GUINEA-PIG SUPERIOR COLLICULUS [J].
ARAKAWA, T ;
OKADA, Y .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1988, 158 (03) :217-224
[2]   GAMMA-AMINOBUTYRIC ACIDA RECEPTOR HETEROGENEITY IS INCREASED BY ALTERNATIVE SPLICING OF A NOVEL BETA-SUBUNIT GENE TRANSCRIPT [J].
BATESON, AN ;
LASHAM, A ;
DARLISON, MG .
JOURNAL OF NEUROCHEMISTRY, 1991, 56 (04) :1437-1440
[3]   GABA(C) RECEPTORS [J].
BORMANN, J ;
FEIGENSPAN, A .
TRENDS IN NEUROSCIENCES, 1995, 18 (12) :515-519
[4]   ELECTROPHYSIOLOGY OF GABAA AND GABAB RECEPTOR SUBTYPES [J].
BORMANN, J .
TRENDS IN NEUROSCIENCES, 1988, 11 (03) :112-116
[5]  
BROWN AG, 1982, Q J EXP PHYSIOL CMS, V67, P193
[6]   Kainate receptors mediate a slow postsynaptic current in hippocampal CA3 neurons [J].
Castillo, PE ;
Malenka, RC ;
Nicoll, RA .
NATURE, 1997, 388 (6638) :182-186
[7]   IDENTIFICATION OF A PUTATIVE GAMMA-AMINOBUTYRIC-ACID (GABA) RECEPTOR SUBUNIT RHO(2) CDNA AND COLOCALIZATION OF THE GENES ENCODING RHO(2) (GABRR2) AND RHO(1) (GABRR1) TO HUMAN-CHROMOSOME 6Q14-Q21 AND MOUSE CHROMOSOME-4 [J].
CUTTING, GR ;
CURRISTIN, S ;
ZOGHBI, H ;
OHARA, B ;
SELDIN, MF ;
UHL, GR .
GENOMICS, 1992, 12 (04) :801-806
[8]   CLONING OF THE GAMMA-AMINOBUTYRIC-ACID (GABA) RHO-1 CDNA - A GABA RECEPTOR SUBUNIT HIGHLY EXPRESSED IN THE RETINA [J].
CUTTING, GR ;
LU, L ;
OHARA, BF ;
KASCH, LM ;
MONTROSERAFIZADEH, C ;
DONOVAN, DM ;
SHIMADA, S ;
ANTONARAKIS, SE ;
GUGGINO, WB ;
UHL, GR ;
KAZAZIAN, HH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (07) :2673-2677
[9]   EXPRESSION OF GABA RECEPTOR RHO-1 AND RHO-2 SUBUNITS IN THE RETINA AND BRAIN OF THE RAT [J].
ENZ, R ;
BRANDSTATTER, JH ;
HARTVEIT, E ;
WASSLE, H ;
BORMANN, J .
EUROPEAN JOURNAL OF NEUROSCIENCE, 1995, 7 (07) :1495-1501
[10]   PHARMACOLOGY OF GABA RECEPTOR CL- CHANNELS IN RAT RETINAL BIPOLAR CELLS [J].
FEIGENSPAN, A ;
WASSLE, H ;
BORMANN, J .
NATURE, 1993, 361 (6408) :159-162