Spectroscopy on single light-harvesting complexes at low temperature

被引：77

作者：

Tietz, C ^{[1
]}

Chekhlov, O ^{[1
]}

Dräbenstedt, A ^{[1
]}

Schuster, J ^{[1
]}

Wrachtrup, J ^{[1
]}

机构：

[1] TU Chemnitz, Inst Phys, D-09107 Chemnitz, Germany

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 1999年 / 103卷 / 30期

关键词：

D O I：

10.1021/jp983599m

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The fluorescence of individual light-harvesting 2 complexes from Rhodopseudomonas acidophila has been observed by confocal microscopy in a temperature range between 300 and 7 K. Under ambient conditions, changes in the polarization of the fluorescence emission of single complexes on a time scale from milliseconds to seconds are found. In the temperature range between 250 and 100 K most complexes emit fluorescence with a temporally stable linear fluorescence polarization. At temperatures below 70 K, spectral diffusion is found to dominate the dynamics of the fluorescence intensity and polarization. The increase in photostability of single complexes at low temperature allows the detection of fluorescence emission spectra of single complexes. A marked variation in the shape and the position of the spectra is found. The results are discussed by considering static and dynamic disorder within the B850 aggregate of the light-harvesting complexes.

引用

页码：6328 / 6333

页数：6

共 32 条

[1] Calculations of spectroscopic properties of the LH2 bacteriochlorophyll - Protein antenna complex from Rhodopseudomonas acidophila [J].