Peri-Golgi vesicles contain retrograde but not anterograde proteins consistent with the cisternal progression model of intra-Golgi transport

被引:133
作者
Martínez-Menárguez, JA
Prekeris, R
Oorschot, VMJ
Scheller, R
Slot, JW
Geuze, HJ
Klumperman, J
机构
[1] Univ Murcia, Sch Med, Dept Cell Biol, Murcia 30071, Spain
[2] Stanford Univ, Sch Med, Howard Hughes Med Inst, Dept Cellular & Mol Physiol, Stanford, CA 94305 USA
[3] Univ Med Ctr Utrecht, Dept Cell Biol, Biomembranes Inst, NL-3584 CX Utrecht, Netherlands
[4] Ctr Biomed Genet, NL-3584 CX Utrecht, Netherlands
关键词
mannosidase II; COP coats; giantin; KDELr; Golgi complex;
D O I
10.1083/jcb.200108029
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
A cisternal progression mode of intra-Golgi transport requires that Golgi resident proteins recycle by peri-Golgi vesicles, whereas the alternative model of vesicular transport predicts anterograde cargo proteins to be present in such vesicles. We have used quantitative immuno-EM on NRK cells to distinguish peri-Golgi vesicles from other vesicles in the Golgi region. We found significant levels of the Golgi resident enzyme mannosidase II and the transport machinery proteins giantin, KDEL-receptor, and rBet1 in coatomer protein 1-coated cisternal rims and peri-Golgi vesicles. By contrast, when cells expressed vesicular stomatitis virus protein G this anterograde marker was largely absent from the peri-Golgi vesicles. These data suggest a role of peri-Golgi vesicles in recycling of Golgi residents, rather than an important role in anterograde transport.
引用
收藏
页码:1213 / 1224
页数:12
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