Rapid one-step purification of the BChl-α containing FMO-protein from the green sulfur bacterium Chlorobium tepidum using a high efficiency immunomatrix

被引：3

作者：

Hu, DD

Blankenship, RE ^{[1
]}

机构：

[1] Arizona State Univ, Dept Chem & Biochem, Tempe, AZ 85287 USA

[2] Arizona State Univ, Ctr Study Early Events Photosynth, Tempe, AZ 85287 USA

来源：

PHOTOSYNTHESIS RESEARCH | 2002年 / 71卷 / 1-2期

关键词：

antibody; bacteriochlorophyll alpha protein; Chlorobium tepidum; chlorosome; FMO-protein; energy transfer; green sulfur bacteria; immunoglobulin;

D O I：

10.1023/A:1014907731595

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A new and rapid procedure has been developed for the isolation of the bacteriochlorophyll a-containing Fenna-Matthews-Olson (FMO)-protein from green sulfur bacteria. Polyclonal antibodies raised against the FMO-protein of Chlorobium (Chl) tepidum were employed in the preparation of an antibody column utilizing immobilized protein A as the matrix. The antibody column afforded essentially a one-step purification process, resulting in preparations that were free from contaminating pigments and proteins. This was evidenced by absorption spectroscopy, SDS-PAGE, and fluorescence emission.

引用

页码：149 / 154

页数：6

共 31 条

[1]

Blankenship R.E., 1995, Anoxygenic photosynthetic bacteria, P399, DOI [DOI 10.1007/0-306-47954-0, 10.1007/0-306-47954-0]

[2] REDOX REGULATION OF ENERGY-TRANSFER EFFICIENCY IN ANTENNAS OF GREEN PHOTOSYNTHETIC BACTERIA [J].