Preparation and characterization of cationic microspheres for gene delivery

被引：38

作者：

Esposito, E

Sebben, S

Cortesi, R

Menegatti, E

Nastruzzi, C

机构：

[1] Univ Perugia, Inst Chim & Technol Farmaco, I-06100 Perugia, Italy

[2] Univ Ferrara, Dipartimento Sci Farmaceut, I-44100 Ferrara, Italy

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 1999年 / 189卷 / 01期

关键词：

cationic microparticles; methacrylic copolymer; controlled release; gene therapy; defibrotide (DFT);

D O I：

10.1016/S0378-5173(99)00231-8

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The production and characterization of cationic microparticles based on Eudragit RS and cationic agents (i.e. a cationic acrylic polymer and three different cationic surfactants) for the delivery of nucleic acids is here described. It was found that morphological and dimensional characteristics of microparticles were influenced by the type and concentration of cationic agent employed and by some experimental parameters such as stirring speed, emulsifying agent and type of rotor. The desoxiribonucleotide Defibrotide (DFT) was associated with positively charged microparticles and its in vitro release kinetics from microparticles were determined. A study of the in vitro toxicity of cationic microparticles on cultured human cell line K562 was also performed, demonstrating that DDAB(18) microparticles display very low cytotoxicity. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：29 / 41

页数：13

共 20 条

[1] SYNTHETIC GENE-TRANSFER VECTORS [J].