IgE detection to α/β/γ-gliadin and its clinical relevance in wheat-dependent exercise-induced anaphylaxis

被引:77
作者
Hofmann, S. C. [1 ]
Fischer, J. [2 ]
Eriksson, C. [3 ]
Gref, O. Bengtsson [3 ]
Biedermann, T. [2 ]
Jakob, T. [1 ]
机构
[1] Univ Med Ctr Freiburg, Dept Dermatol, Allergy Res Grp, D-79104 Freiburg, Germany
[2] Univ Tubingen Hosp, Dept Dermatol, Allergy Unit, D-72076 Tubingen, Germany
[3] Thermo Fisher Sci, Uppsala, Sweden
关键词
gliadin; IgE; recombinant allergens; wheat allergy; wheat-dependent exercise-induced anaphylaxis; OMEGA-5; GLIADIN; DIAGNOSIS; GLUTENIN;
D O I
10.1111/all.12020
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Wheat-dependent exercise-induced anaphylaxis (WDEIA) is characterized by anaphylactic reactions after wheat ingestion and physical exercise. IgE antibodies to recombinant ?5-gliadin are detectable in a majority of WDEIA patients, but other wheat allergens may also play a role in elicitation of WDEIA. Here, we performed a comprehensive analysis of IgE reactivity to different wheat proteins in 17 patients with confirmed WDEIA by ImmunoCAP research prototypes and a semi-quantitative microarray immunoassay with a/beta/?-gliadin, high-molecular-weight (HMW) glutenin, alpha-amylase inhibitor (AAI) dimer, and wheat lipid transfer protein (LTP). By ImmunoCAP, IgE to recombinant ?5-gliadin was detectable in 14/17 patients (82%), to a/beta/?-gliadin in 82% including the three patients lacking IgE to ?5-gliadin, and to HMW glutenin in 59%. The microarray revealed specifically ?-gliadin as the second most important allergen. These results demonstrate the additional diagnostic value of a/beta- and ?-gliadin in particular in ?5-gliadin-negative patients in the diagnosis of WDEIA.
引用
收藏
页码:1457 / 1460
页数:4
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