Toxin III from Leiurus quinquestriatus quinquestriatus: A specific probe for receptor site 3 on insect sodium channels

被引：13

作者：

Cestele, S ^{[1
]}

Gordon, D ^{[1
]}

Kopeyan, C ^{[1
]}

Rochat, H ^{[1
]}

机构：

[1] UNIV MEDITERANNEE,LAB BIOCHIM IMPENIERIE PROT UNR6560,CNRS IFR JEAN ROCHE,F-13916 MARSEILLE 20,FRANCE

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1997年 / 27卷 / 06期

关键词：

insect sodium channel; scorpion toxin;

D O I：

10.1016/S0965-1748(97)00027-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Scorpion toxin Lqq III binds to a single class of high affinity (K-d = 72 +/- 19 pM) and low capacity (B-max= 2.5 +/- 0.2 pmol/mg) binding sites in cockroach neuronal membranes, Its binding was inhibited by Lqh alpha IT (IC50 = 80 +/- 30 pM) and sea-anemone toxin ATX II (IC50 = 2.5 +/- 0.3 nM), suggesting that Lqq III is a specific probe for receptor site 3 on cockroach sodium channels. This was confirmed by competitive binding experiments between I-125-Lqq III and scorpion alpha-toxins which have less toxicity in insects, (C) 1997 Elsevier Science Ltd.

引用

页码：523 / 528

页数：6

共 29 条

[1]

Behrens B., 1934, ARCH EXP PATHOL PH, V177, P379

[2]

CATTERALL WA, 1978, J BIOL CHEM, V253, P7393

[3]

CATTERALL WA, 1977, J BIOL CHEM, V252, P8669

[4]

CATTERALL WA, 1992, PHYSIOL REV, V72, P15