Zinc is required in pyrrolidine dithiocarbamate inhibition of NF-κB activation

Pyrrolidine dithiocarbamate (PDTC) is a potent inhibitor of nuclear factor kappa B (NF-kappa B) activation. PDTC inhibited basal IVF-kappa B activity of endothelial cells. PDTC, however, failed to inhibit basal NF-kappa B activity after,withdrawal of serum in the media, and the inhibitory effect of PDTC could be restored by addition of tint. When various preparations of metal ion-EDTA were tested,vith PDTC in serum-containing media, only Zn-EDTA failed to block the inhibitory effect of PDTC, The dependence on zinc was also noted in PDTC inhibition of NF-kappa B stimulated by TNF alpha. These facts suggest that zinc is required for PDTC inhibition of NF-kappa B activation. (C) 1999 Federation of European Biochemical Societies.