Characterization of adhesin variants in Indian isolates of enteroaggregative Escherichia coli

被引：12

作者：

Bhardwaj, R

Majumdar, S

Ganguly, NK

Taneja, N

Dutta, S

Ramamurthy, T

Chakraborti, A ^{[1
]}

机构：

[1] Postgrad Inst Med Educ & Res, Dept Expt Med & Biotechnol, Chandigarh 160012, India

[2] Postgrad Inst Med Educ & Res, Dept Med Microbiol, Chandigarh 160012, India

[3] Natl Inst Cholera & Enter Dis, Kolkata, W Bengal, India

[4] Indian Council Med Res, New Delhi, India

来源：

FEMS MICROBIOLOGY LETTERS | 2006年 / 258卷 / 02期

基金：

欧盟地平线“2020”;

关键词：

diarrhea; Escherichia coli; EAEC; AAF; aggregative adherence phenotype; HEp-2 adherence assay;

D O I：

10.1111/j.1574-6968.2006.00231.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Enteroaggregative Escherichia coli (EAEC) are causative agents of diarrhea, being characterized by aggregative adherence to cultured epithelial cells. In this study, phenotypic properties of EAEC were analyzed with respect to AA, hemagglutination, clump and biofilm formation, all of which are mediated by aggregative adherence fimbriae (AAF). The strains were also screened for AAF types, AAF adhesin variants and Dr adhesin by PCR. Of the three known AAF types, AAF/I and AAF/II adhesin variants were identified. An association between the AAF/adhesin genotypes and the subtypes/scores of phenotypic properties was sought and it was observed that strains harboring same adhesins displayed different subtypes/scores and vice versa.

引用

收藏

页码：274 / 283

页数：10

相关论文

共 38 条

[1] BACTERIAL CLUMP FORMATION AT THE SURFACE OF LIQUID CULTURE AS A RAPID TEST FOR IDENTIFICATION OF ENTEROAGGREGATIVE ESCHERICHIA-COLI [J].

ALBERT, MJ ;

QADRI, F ;

HAQUE, A ;

BHUIYAN, NA .

JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (05) :1397-1399

[2] Identification of an aggregative adhesion fimbria (AAF) type III-encoding operon in enteroaggregative Escherichia coli as a sensitive probe for detecting the AAF-Encoding operon family [J].

Bernier, C ;

Gounon, P ;

Le Bouguénec, C .

INFECTION AND IMMUNITY, 2002, 70 (08) :4302-4311

[3]

BEUTIN L, 1991, MED MICROBIOL IMMUN, V180, P167

[4] ENTEROAGGREGATIVE ESCHERICHIA-COLI ASSOCIATED WITH PERSISTENT DIARRHEA IN A COHORT OF RURAL CHILDREN IN INDIA [J].

BHAN, MK ;

RAJ, P ;

LEVINE, MM ;

KAPER, JB ;

BHANDARI, N ;

SRIVASTAVA, R ;

KUMAR, R ;

SAZAWAL, S .

JOURNAL OF INFECTIOUS DISEASES, 1989, 159 (06) :1061-1064

[5] Concomitant infection of enterotoxigenic Escherichia coli in an outbreak of cholera caused by Vibrio cholerae O1 and O139 in Ahmedabad, India [J].

Chakraborty, S ;

Deokule, JS ;

Garg, P ;

Bhattacharya, SK ;

Nandy, RK ;

Nair, GB ;

Yamasaki, S ;

Takeda, Y ;

Ramamurthy, T .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (09) :3241-3246

[6] ADHESIVE FACTOR FOUND IN STRAINS OF ESCHERICHIA-COLI BELONGING TO THE TRADITIONAL INFANTILE ENTEROPATHOGENIC SEROTYPES [J].

CRAVIOTO, A ;

GROSS, RJ ;

SCOTLAND, SM ;

ROWE, B .

CURRENT MICROBIOLOGY, 1979, 3 (02) :95-99

[7] Aggregative adherence fimbria II, a second fimbrial antigen mediating aggregative adherence in enteroaggregative Escherichia coli [J].

Czeczulin, JR ;

Balepur, S ;

Hicks, S ;

Phillips, A ;

Hall, R ;

Kothary, MH ;

NavarroGarcia, F ;

Nataro, JP .

INFECTION AND IMMUNITY, 1997, 65 (10) :4135-4145

[8] Phylogenetic analysis of enteroaggregative and diffusely adherent Escherichia coli [J].

Czeczulin, JR ;

Whittam, TS ;

Henderson, IR ;

Navarro-Garcia, F ;

Nataro, JP .

INFECTION AND IMMUNITY, 1999, 67 (06) :2692-2699

[9] Use of PCR to identify enteroaggregative Escherichia coli as an important cause of acute diarrhoea among children living in Calcutta, India [J].

Dutta, S ;

Pal, S ;

Chakrabarti, S ;

Dutta, P ;

Manna, B .

JOURNAL OF MEDICAL MICROBIOLOGY, 1999, 48 (11) :1011-1016

[10] Distribution of aggA and aafA gene sequences among Escherichia coli isolates with genotypic or phenotypic characteristics, or both, of enteroaggregative E-coli [J].

Elias, WP ;

Suzart, S ;

Trabulsi, LR ;

Nataro, JP ;

Gomes, TAT .

JOURNAL OF MEDICAL MICROBIOLOGY, 1999, 48 (06) :597-599

← 1 2 3 4 →