Simultaneous detection of human enterovirus 71 and coxsackievirus A16 in clinical specimens by multiplex real-time PCR with an internal amplification control

被引：62

作者：

Xiao, Xing-Long ^{[1
,3
]}

He, Ya-Qing ^{[2
]}

Yu, Yi-Gang ^{[1
]}

Yang, Hong ^{[2
]}

Chen, Gu ^{[1
]}

Li, Hui-Fang ^{[3
]}

Zhang, Jing-Wei ^{[3
]}

Liu, Dong-Mei ^{[1
]}

Li, Xiao-Feng ^{[1
]}

Yang, Xiao-Quan ^{[1
]}

Wu, Hui ^{[1
]}

机构：

[1] S China Univ Technol, Coll Light Ind & Food, Dept Food, Inst Food Safety, Guangzhou 510640, Guangdong, Peoples R China

[2] Shenzhen Ctr Dis Control & Prevent, Shenzhen 518000, Peoples R China

[3] Shenzhen Taitai Genom Inc, Shenzhen 518057, Peoples R China

来源：

ARCHIVES OF VIROLOGY | 2009年 / 154卷 / 01期

基金：

中国国家自然科学基金;

关键词：

DIAGNOSIS; VP1;

D O I：

10.1007/s00705-008-0266-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The recent and continuing HFMD outbreak caused by EV71 in several provinces of China since March 2008 has affected thousands of children and resulted in nearly 50 deaths. In this study, a sensitive and specific multiplex real-time RT-PCR assay has been developed for the rapid detection of EV71 and CV-A16. By using an internal amplification control, the real-time assay achieves detection of samples containing inhibitors and avoids false negatives. It should prove useful for clinical diagnosis of EV71 or CV-A16 infections.

引用

页码：121 / 125

页数：5

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