Degradable bisphosphonate-alkaline phosphatase-complexed hydroxyapatite implants in vitro

被引:28
作者
Denissen, H
VanBeek, E
VandenBos, T
DeBlieck, J
Klein, C
VandenHooff, A
机构
[1] UNIV LEIDEN HOSP, DEPT ENDOCRINOL & METAB DIS, NL-2300 RC LEIDEN, NETHERLANDS
[2] ACAD CTR DENT AMSTERDAM, DEPT PERIODONTOL, NL-1066 EA AMSTERDAM, NETHERLANDS
[3] UNIV AMSTERDAM, ACAD MED CTR, DEPT CELL BIOL & HISTOL, NL-1105 AZ AMSTERDAM, NETHERLANDS
关键词
D O I
10.1359/jbmr.1997.12.2.290
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Degradable hydroxyapatite (HA) implants complexed with the resorption inhibiting agent bisphosphonate (PCP) and the mineralizing agent alkaline phosphatase (ALP) can theoretically maintain alveolar bone mass directly after extraction of teeth, The present in vitro study investigated the surface properties of PCP-ALP-complexed HA implants in relation to the requirements of implant behavior and action, Adsorbed PCP (pH 3.49) resulted in a flattening and broadening of the phosphate peaks and the formation of carbonate peaks in the HA pattern of the implant indicating a chemical alteration of the HA surface, Adsorption of ALP onto PCP-altered HA surfaces was 26% lower than onto HA implant blank surfaces, PCP-ALP-complexed HA implants released the PCP and ALP steadily and continuously over observation periods of, respectively, 75 and 14 days, During these observation periods, the ceramic grains of the HA implant became smaller and intergrain boundaries became broader, These morphologic characteristics suggested preconditioning of the HA implant surface for future bonding and degradation in vivo., Individual grains were no longer bonded to other grains and detached from the implant which had become rounded in shape, From in vitro mice experiments we found that PCP concentrations between 10(-4) and 10(-3) M resulted in Ca-45-release from the bone HA. Our calculations showed, however, that only a total concentration of 1.4 x 10(-4) M PCP was gradually released over the whole observation period, In another experiment, it appeared that a PCP concentration in solution <10(-3) M did not reduce ALP activity, It is concluded that release of PCP by the PCP-ALP-complexed implants is maintained at levels in the range to impair osteoclast bone resorption but not high enough to block osteoblast activity, The amount of ALP released can lead to induction of bone formation onto implant surfaces, pH-induced alterations in the microstructure and chemistry of the HA surface allow for controlled degradation of the HA implants in vitro, A PCP-ALP-complexed HA implant acting as temporary scaffolding for alveolar bone growth enhancement, mineralization, and maintenance seems to be a reasonable concept for preservation of the edentulous alveolus.
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页码:290 / 297
页数:8
相关论文
共 48 条
[1]   REDUCTION OF RESIDUAL RIDGES - MAJOR ORAL DISEASE ENTITY [J].
ATWOOD, DA .
JOURNAL OF PROSTHETIC DENTISTRY, 1971, 26 (03) :266-+
[2]  
Becker W, 1994, Int J Oral Maxillofac Implants, V9, P31
[3]   GUIDED TISSUE REGENERATION FOR IMPLANTS PLACED INTO EXTRACTION SOCKETS - A STUDY IN DOGS [J].
BECKER, W ;
BECKER, BE ;
HANDELSMAN, M ;
OCHSENBEIN, C ;
ALBREKTSSON, T .
JOURNAL OF PERIODONTOLOGY, 1991, 62 (11) :703-709
[4]   ALKALINE-PHOSPHATASE INDUCES THE DEPOSITION OF CALCIFIED LAYERS IN RELATION TO DENTIN - AN INVITRO STUDY TO MIMIC THE FORMATION OF AFIBRILLAR ACELLULAR CEMENTUM [J].
BEERTSEN, W ;
VANDENBOS, T .
JOURNAL OF DENTAL RESEARCH, 1991, 70 (03) :176-181
[5]  
BOONEKAMP PM, 1986, BONE MINER, V1, P27
[6]  
Branemark P I, 1977, Scand J Plast Reconstr Surg Suppl, V16, P1
[7]  
BRANEMARK P I, 1969, Scandinavian Journal of Plastic and Reconstructive Surgery, V3, P81
[8]  
Buser D, 1993, Int J Periodontics Restorative Dent, V13, P29
[9]   A CLASSIFICATION OF THE EDENTULOUS JAWS [J].
CAWOOD, JI ;
HOWELL, RA .
INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, 1988, 17 (04) :232-236
[10]   FORMATION OF CARBONATE-APATITE CRYSTALS AFTER IMPLANTATION OF CALCIUM-PHOSPHATE CERAMICS [J].
DACULSI, G ;
LEGEROS, RZ ;
HEUGHEBAERT, M ;
BARBIEUX, I .
CALCIFIED TISSUE INTERNATIONAL, 1990, 46 (01) :20-27