Development of a monoclonal antibody blocking-ELISA for detection of antibodies against Maedi-Visna virus

被引：13

作者：

Fevereiro, M ^{[1
]}

Barros, S ^{[1
]}

Fagulha, T ^{[1
]}

机构：

[1] Lab Nacl Invest Vet, Dept Virol, Lisbon, Portugal

来源：

JOURNAL OF VIROLOGICAL METHODS | 1999年 / 81卷 / 1-2期

关键词：

monoclonal antibody; Maedi-Visna virus; Blck-ELISA;

D O I：

10.1016/S0166-0934(99)00061-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A monoclonal antibody (MAb) blocking ELISA (Blck-ELISA) was developed to detect antibodies against Maedi-Visna virus (MW) in sheep sera. The assay employs a MAb directed against the envelope protein p90 of the virus in a sandwich blocking procedure. To determine whether the MAb was a potential antibody for developing a Blck-ELISA, a collection of three hundred sera obtained from several sheep flocks known to be infected with MW were used to examine the sensitivity of the Blck-ELISA. A total of 50 serum samples originating from a flock free of MW were tested to assess the specificity of the assay. The results were compared with a commercial indirect ELISA (I-ELISA) and samples giving a conflicting or doubtful result were tested by immunoblot. The Blck-ELISA proved to be specific, sensitive and it showed high reproducibility and low variability. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：101 / 108

页数：8

共 22 条

[1] Detection of Maedi-Visna virus antibodies using a single fusion transmembrane-core p25 recombinant protein ELISA and a modified receiver-operating characteristic analysis to determine cut-off values [J].