Expression profiling reveals metabolic and structural components of extraocular muscles

被引:94
作者
Fischer, MD
Gorospe, JR
Felder, E
Bogdanovich, S
Pedrosa-Domellöf, F
Ahima, RS
Rubinstein, NA
Hoffman, EP
Khurana, TS
机构
[1] Univ Penn, Sch Med, Dept Physiol, Philadelphia, PA 19104 USA
[2] Univ Penn, Sch Med, Penn Muscle Inst, Philadelphia, PA 19104 USA
[3] George Washington Univ, Childrens Natl Med Ctr, Med Genet Res Ctr, Washington, DC 20010 USA
[4] Univ Penn, Sch Med, Dept Cell & Dev Biol, Philadelphia, PA 19104 USA
[5] Umea Univ, Sect Anat, Dept Integrat Med Biol, SE-90187 Umea, Sweden
[6] Univ Penn, Sch Med, Div Endocrinol, Philadelphia, PA 19104 USA
关键词
extraocular muscle; allotype; expression profile; transcriptome; energy metabolism;
D O I
10.1152/physiolgenomics.00115.2001
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The extraocular muscles (EOM) are anatomically and physiologically distinct from other skeletal muscles. EOM are preferentially affected in mitochondrial myopathies, but spared in Duchenne's muscular dystrophy. The anatomical and pathophysiological properties of EOM have been attributed to their unique molecular makeup: an allotype. We used expression profiling to define molecular features of the EOM allotype. We found 346 differentially expressed genes in rat EOM compared with tibialis anterior, based on a twofold difference cutoff. Genes required for efficient, fatigue-resistant, oxidative metabolism were increased in EOM, whereas genes for glycogen metabolism were decreased. EOM also showed increased expression of genes related to structural components of EOM such as vessels, nerves, mitochondria, and neuromuscular junctions. Additionally, genes related to specialized functional roles of EOM such as the embryonic and EOM-specific myosin heavy chains and genes for muscle growth, development, and/or regeneration were increased. The EOM expression profile was validated using biochemical, structural, and molecular methods. Characterization of the EOM expression profile begins to define gene transcription patterns associated with the unique anatomical, metabolic, and pathophysiological properties of EOM.
引用
收藏
页码:71 / 84
页数:14
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共 50 条
  • [1] ASMUSSEN G, 1978, ACTA BIOL MED GER, V37, P301
  • [2] CHARACTERIZATION OF THE SARCOPLASMIC-RETICULUM PROTEINS IN THE THERMOGENIC MUSCLES OF FISH
    BLOCK, BA
    OBRIEN, J
    MEISSNER, G
    [J]. JOURNAL OF CELL BIOLOGY, 1994, 127 (05) : 1275 - 1287
  • [3] Bron AJ., 1997, WOLFFS ANATOMY EYE O, V8th
  • [4] MOLECULAR-BIOLOGY OF DUCHENNE MUSCULAR-DYSTROPHY
    BROWN, RH
    HOFFMAN, EP
    [J]. TRENDS IN NEUROSCIENCES, 1988, 11 (11) : 480 - 484
  • [5] Brown SE, 1997, DYSTROPHIN GENE PROT
  • [6] Brueckner JK, 1996, J MUSCLE RES CELL M, V17, P297
  • [7] Altered kinetics of contraction of mouse atrial myocytes expressing ventricular myosin regulatory light chain
    Buck, SH
    Konyn, PJ
    Palermo, J
    Robbins, J
    Moss, RL
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 1999, 276 (04): : H1167 - H1171
  • [8] Expression profiling in the muscular dystrophies: Identification of novel aspects of molecular pathophysiology
    Chen, YW
    Zhao, P
    Borup, R
    Hoffman, EP
    [J]. JOURNAL OF CELL BIOLOGY, 2000, 151 (06) : 1321 - 1336
  • [9] Christiansen SP, 2000, INVEST OPHTH VIS SCI, V41, P3402
  • [10] Running exercise may reduce risk for lung and liver cancer by inducing activity of antioxidant and phase II enzymes
    Duncan, K
    Harris, S
    Ardies, CM
    [J]. CANCER LETTERS, 1997, 116 (02) : 151 - 158