Combined linkage and association mapping reveals CYCD5;1 as a quantitative trait gene for endoreduplication in Arabidopsis

被引：44

作者：

Sterken, Roel ^{[1
,2
]}

Kiekens, Raphael ^{[1
,2
]}

Boruc, Joanna ^{[1
,2
]}

Zhang, Fanghong ^{[3
]}

Vercauteren, Annelies ^{[1
,2
]}

Vercauteren, Ilse ^{[1
,2
]}

De Smet, Lien ^{[1
,2
]}

Dhondt, Stijn ^{[1
,2
]}

Inze, Dirk ^{[1
,2
]}

De Veylder, Lieven ^{[1
,2
]}

Russinova, Eugenia ^{[1
,2
]}

Vuylsteke, Marnik ^{[1
,2
]}

机构：

[1] VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium

[2] Univ Ghent, Dept Biotechnol & Bioinformat, B-9052 Ghent, Belgium

[3] Univ Ghent, Dept Appl Math & Comp Sci, B-9000 Ghent, Belgium

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2012年 / 109卷 / 12期

关键词：

change-of-function allele; endopolyploidy; regulatory haplotype; CYCLIN-DEPENDENT KINASE; MAYS SSP PARVIGLUMIS; GENOME-WIDE ANALYSIS; CANDIDATE GENE; CELL-SIZE; PLANT DEVELOPMENT; LEAF DEVELOPMENT; ENDOCYCLE ONSET; ORGAN GROWTH; THALIANA;

D O I：

10.1073/pnas.1120811109

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Endoreduplication is the process where a cell replicates its genome without mitosis and cytokinesis, often followed by cell differentiation. This alternative cell cycle results in various levels of endoploidy, reaching 4x or higher one haploid set of chromosomes. Endoreduplication is found in animals and is widespread in plants, where it plays a major role in cellular differentiation and plant development. Here, we show that variation in endoreduplication between Arabidopsis thaliana accessions Columbia-0 and Kashmir is controlled by two major quantitative trait loci, ENDO-1 and ENDO-2. A local candidate gene association analysis in a set of 87 accessions, combined with expression analysis, identified CYCD5;1 as the most likely candidate gene underlying ENDO-2, operating as a rate-determining factor of endoreduplication. In accordance, both the overexpression and silencing of CYCD5;1 were effective in changing DNA ploidy levels, confirming CYCD5;1 to be a previously undescribed quantitative trait gene underlying endoreduplication in Arabidopsis.

引用

页码：4678 / 4683

页数：6

共 59 条

[1] Programmed induction of endoreduplication by DNA double-strand breaks in Arabidopsis [J].

Adachi, Sumiko ;

Minamisawa, Kazunori ;

Okushima, Yoko ;

Inagaki, Soichi ;

Yoshiyama, Kaoru ;

Kondou, Youichi ;

Kaminuma, Eli ;

Kawashima, Mika ;

Toyoda, Tetsuro ;

Matsui, Minami ;

Kurihara, Daisuke ;

Matsunaga, Sachihiro ;

Umeda, Masaaki .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (24) :10004-10009

[2] Genetic and molecular analyses of natural variation indicate CBF2 as a candidate gene for underlying a freezing tolerance quantitative trait locus in Arabidopsis [J].

Alonso-Blanco, C ;

Gomez-Mena, C ;

Llorente, F ;

Koornneef, M ;

Salinas, J ;

Martínez-Zapater, JM .

PLANT PHYSIOLOGY, 2005, 139 (03) :1304-1312

[3] What Has Natural Variation Taught Us about Plant Development, Physiology, and Adaptation? [J].

Alonso-Blanco, Carlos ;

Aarts, Mark G. M. ;

Bentsink, Leonie ;

Keurentjes, Joost J. B. ;

Reymond, Matthieu ;

Vreugdenhil, Dick ;

Koornneef, Maarten .

PLANT CELL, 2009, 21 (07) :1877-1896

[4] Exit from Proliferation during Leaf Development in Arabidopsis thaliana: A Not-So-Gradual Process [J].

Andriankaja, Megan ;

Dhondt, Stijn ;

De Bodt, Stefanie ;

Vanhaeren, Hannes ;

Coppens, Frederik ;

De Milde, Liesbeth ;

Muehlenbock, Per ;

Skirycz, Aleksandra ;

Gonzalez, Nathalie ;

Beemster, Gerrit T. S. ;

Inze, Dirk .

DEVELOPMENTAL CELL, 2012, 22 (01) :64-78

[5] Genome-wide association study of 107 phenotypes in Arabidopsis thaliana inbred lines [J].

Atwell, Susanna ;

Huang, Yu S. ;

Vilhjalmsson, Bjarni J. ;

Willems, Glenda ;

Horton, Matthew ;

Li, Yan ;

Meng, Dazhe ;

Platt, Alexander ;

Tarone, Aaron M. ;

Hu, Tina T. ;

Jiang, Rong ;

Muliyati, N. Wayan ;

Zhang, Xu ;

Amer, Muhammad Ali ;

Baxter, Ivan ;

Brachi, Benjamin ;

Chory, Joanne ;

Dean, Caroline ;

Debieu, Marilyne ;

de Meaux, Juliette ;

Ecker, Joseph R. ;

Faure, Nathalie ;

Kniskern, Joel M. ;

Jones, Jonathan D. G. ;

Michael, Todd ;

Nemri, Adnane ;

Roux, Fabrice ;

Salt, David E. ;

Tang, Chunlao ;

Todesco, Marco ;

Traw, M. Brian ;

Weigel, Detlef ;

Marjoram, Paul ;

Borevitz, Justin O. ;

Bergelson, Joy ;

Nordborg, Magnus .

NATURE, 2010, 465 (7298) :627-631

[6] Patterned cell determination in a plant tissue: the secondary phloem of trees [J].

Barlow, P .

BIOESSAYS, 2005, 27 (05) :533-541

[7] Endopolyploidy in seed plants is differently correlated to systematics, organ, life strategy and genome size [J].

Barow, M ;

Meister, A .

PLANT CELL AND ENVIRONMENT, 2003, 26 (04) :571-584

[8] Genome-wide analysis of gene expression profiles associated with cell cycle transitions in growing organs of Arabidopsis [J].

Beemster, GTS ;

De Veylder, L ;

Vercruysse, S ;

West, G ;

Rombaut, D ;

Van Hummelen, P ;

Galichet, A ;

Gruissem, W ;

Inzé, D ;

Vuylsteke, M .

PLANT PHYSIOLOGY, 2005, 138 (02) :734-743

[9] Variation in growth rate between Arabidopsis ecotypes is correlated with cell division and A-type cyclin-dependent kinase activity [J].

Beemster, GTS ;

De Vusser, K ;

De Tavernier, E ;

De Bock, K ;

Inzé, D .

PLANT PHYSIOLOGY, 2002, 129 (02) :854-864

[10] The plant-specific cyclin-dependent kinase CDKB1;1 and transcription factor E2Fa-DPa control the balance of mitotically dividing and endoreduplicating cells in Arabidopsis [J].

Boudolf, V ;

Vlieghe, K ;

Beemster, GTS ;

Magyar, Z ;

Acosta, JAT ;

Maes, S ;

Van Der Schueren, E ;

Inzé, D ;

De Veylder, L .

PLANT CELL, 2004, 16 (10) :2683-2692

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