A novel recognition motif of human NKT antigen receptor for a glycolipid ligand

被引：52

作者：

Kawano, T

Tanaka, Y

Shimizu, E

Kaneko, Y

Kamata, N

Sato, H

Osada, H

Sekiya, S

Nakayama, T

Taniguchi, M

机构：

[1] Chiba Univ, CREST, Chuo Ku, Chiba 2608670, Japan

[2] Chiba Univ, Dept Mol Immunol, Chuo Ku, Chiba 2608670, Japan

[3] Chiba Univ, Grad Sch Med, Dept Dev Immunol, Chuo Ku, Chiba 2608670, Japan

[4] Chiba Univ, Sch Med, Dept Obstet & Gynaecol, Chuo Ku, Chiba 2608670, Japan

来源：

INTERNATIONAL IMMUNOLOGY | 1999年 / 11卷 / 06期

关键词：

carbohydrate-binding proteins; complementarity-determining region; NKT antigen receptor; protein-sugar interaction; repertoire selection;

D O I：

10.1093/intimm/11.6.881

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Murine NKT cells can recognize a-galactosylceramide (alpha-GalCer) in the context of a class Ib CD1d molecule. Here we show that alpha-GalCer can selectively activate freshly isolated human V(alpha)24(+)V(beta)11 (+) cells, functionally defining the human NKT cells. The naive human NKT cell repertoire consisted of cells expressing an invariant V(alpha)24J(alpha)Q chain and a diverse array of beta chains derived from a single V beta 11 gene segment. Stimulation with a-GalCer expanded a polyclonal subset of the human NKT cell repertoire carrying a novel complementarity-determining region (CDR) 3 beta consensus motif that may directly interact with the sugar moiety of a-GalCer, Our data suggest that certain redundancy is allowed for CDR3 beta of NKT antigen receptor to interact with the ligand and provide a first clue to understand the novel protein-carbohydrate interaction mechanisms.

引用

页码：881 / 887

页数：7

共 23 条

[1] Requirement for V(alpha)14 NKT cells in IL-12-mediated rejection of tumors [J].