In Vitro Reconstitution of the Radical S-Adenosylmethionine Enzyme MqnC Involved in the Biosynthesis of Futalosine-Derived Menaquinone

被引：38

作者：

Cooper, Lisa E. ^{[1
]}

Fedoseyenko, Dmytro ^{[1
]}

Abdelwahed, Sameh H. ^{[1
]}

Kim, Soong-Hyun ^{[1
]}

Dairi, Tohru ^{[2
]}

Begley, Tadhg P. ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Chem, College Stn, TX 77842 USA

[2] Hokkaido Univ, Grad Sch Engn, Sapporo, Hokkaido, Japan

来源：

BIOCHEMISTRY | 2013年 / 52卷 / 27期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1021/bi400498d

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The radical S-adenosylmethionine enzyme MqnC catalyzes conversion of dehypoxanthine futalosine (DHFL) to the unique Spiro compound cyclic DHFL in the futalosine pathway for menaquinone biosynthesis. This study describes the in vitro reconstitution of [4Fe-4S] cluster-dependent MqnC activity and identifies the site of abstraction of a hydrogen atom from DHFL by the adenosyl radical.

引用

页码：4592 / 4594

页数：3

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