Cutting Edge: Developmental Stage-Specific Recruitment of Cohesin to CTCF Sites throughout Immunoglobulin Loci during B Lymphocyte Development

被引:127
作者
Degner, Stephanie C. [1 ]
Wong, Timothy P. [1 ]
Jankevicius, Gytis [1 ]
Feeney, Ann J. [1 ]
机构
[1] Scripps Res Inst, Dept Immunol & Microbial Sci, La Jolla, CA 92037 USA
基金
美国国家卫生研究院;
关键词
HEAVY-CHAIN LOCUS; HUMAN GENOME; CELL DEVELOPMENT; CHROMATIN; INSULATOR; GENE; IKAROS;
D O I
10.4049/jimmunol.182.1.44
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Contraction of the large Igh and Ig kappa loci brings all V genes, spanning > 2.5 Mb in each locus, in proximity to DJ(H) or J(kappa) genes. CCCTC-binding factor (CTCF) is a transcription factor that regulates gene expression by long-range chromosomal looping. We therefore hypothesized that CTCF may be crucial for the contraction of the Ig loci, but no CTCF sites have been described in any V loci. Using ChIP-chip, we demonstrated many CTCF sites in the V-H and V-kappa regions. However, CTCF enrichment in the Igh locus, but not the Ig kappa locus, was largely unchanged throughout differentiation, suggesting that CTCF binding alone cannot be responsible for stage-specific looping. Because cohesin can colocalize with CTCF, we performed chromatin immunoprecipitation for the cohesin subunit Rad21 and found lineage and stage-specific Rad21 recruitment to CTCF in all Ig loci. The differential binding of cohesin to CTCF sites may promote multiple loop formation and thus effective V(D)J recombination. The Journal of Immunology, 2009, 182: 44-48.
引用
收藏
页码:44 / 48
页数:5
相关论文
共 24 条
  • [1] High-resolution profiling of histone methylations in the human genome
    Barski, Artern
    Cuddapah, Suresh
    Cui, Kairong
    Roh, Tae-Young
    Schones, Dustin E.
    Wang, Zhibin
    Wei, Gang
    Chepelev, Iouri
    Zhao, Keji
    [J]. CELL, 2007, 129 (04) : 823 - 837
  • [2] Identification of a Ctcf cofactor, Yy1, for the X chromosome binary switch
    Donohoe, Mary E.
    Zhang, Li-Feng
    Xu, Na
    Shi, Yang
    Lee, Jeannie T.
    [J]. MOLECULAR CELL, 2007, 25 (01) : 43 - 56
  • [3] The extent of histone acetylation correlates with the differential rearrangement frequency of individual VH genes in pro-B cells
    Espinoza, CR
    Feeney, AJ
    [J]. JOURNAL OF IMMUNOLOGY, 2005, 175 (10) : 6668 - 6675
  • [4] Chromatin architecture near a potential 3′ end of the Igh locus involves modular regulation of histone modifications during B-Cell development and in vivo occupancy at CTCF sites
    Garrett, FE
    Emelyanov, AV
    Sepulveda, MA
    Flanagan, P
    Volpi, S
    Li, FB
    Loukinov, D
    Eckhardt, LA
    Lobanenkov, VV
    Birshtein, BK
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 2005, 25 (04) : 1511 - 1525
  • [5] CTCF-dependent chromatin insulator is linked to epigenetic remodeling
    Ishihara, Ko
    Oshimura, Mitsuo
    Nakao, Mitsuyoshi
    [J]. MOLECULAR CELL, 2006, 23 (05) : 733 - 742
  • [6] The 3D structure of the immunoglobulin heavy-chain locus: Implications for long-range genomic interactions
    Jhunjhunwala, Suchit
    van Zelm, Menno C.
    Peak, Mandy M.
    Cutchin, Steve
    Riblet, Roy
    van Dongen, Jacques J. M.
    Grosveld, Frank G.
    Knoch, Tobias A.
    Murre, Cornelis
    [J]. CELL, 2008, 133 (02) : 265 - 279
  • [7] Model-based analysis of tiling-arrays for ChIP-chip
    Johnson, W. Evan
    Li, Wei
    Meyer, Clifford A.
    Gottardo, Raphael
    Carroll, Jason S.
    Brown, Myles
    Liu, X. Shirley
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (33) : 12457 - 12462
  • [8] Analysis of the vertebrate insulator protein CTCF-binding sites in the human genome
    Kim, Tae Hoon
    Abdullaev, Ziedulla K.
    Smith, Andrew D.
    Ching, Keith A.
    Loukinov, Dmitri I.
    Green, Roland D.
    Zhang, Michael Q.
    Lobanenkov, Victor V.
    Ren, Bing
    [J]. CELL, 2007, 128 (06) : 1231 - 1245
  • [9] Subnuclear compartmentalization of immunoglobulin loci during lymphocyte development
    Kosak, ST
    Skok, JA
    Medina, KL
    Riblet, R
    Le Beau, MM
    Fisher, AG
    Singh, H
    [J]. SCIENCE, 2002, 296 (5565) : 158 - 162
  • [10] CTCF mediates interchromosomal colocalization between Igf2/H19 and Wsb1/Nf1
    Ling, JQ
    Li, T
    Hu, JF
    Vu, TH
    Chen, HL
    Qiu, XW
    Cherry, AM
    Hoffman, AR
    [J]. SCIENCE, 2006, 312 (5771) : 269 - 272