Utilisation of reversed-phase high-performance liquid chromatography as an alternative to silver-ion chromatography for the separation of cis- and trans-C18:1 fatty acid isomers

被引：56

作者：

Juanéda, P ^{[1
]}

机构：

[1] INRA, Unite Nutr Lipid, F-21065 Dijon, France

来源：

JOURNAL OF CHROMATOGRAPHY A | 2002年 / 954卷 / 1-2期

关键词：

silver-ion chromatography; trans fatty acid methyl esters;

D O I：

10.1016/S0021-9673(02)00179-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Silver ion-high-performance liquid chromatography (HPLC) has been commonly used for the separation and the analysis of trans-18:1 isomers in partially hydrogenated oils and milk fat. This paper describes an easy HPLC method using two reversed-phase columns. The cis- and trans-18:1 fatty acids isomers as methyl esters were eluted as two separate fractions. The collected fractions were analysed by gas chromatography (GC). The purity of the two fractions were tested by GC-MS and GC-Fourier transform IR. (C) 2002 Elsevier Science B V. All rights reserved.

引用

页码：285 / 289

页数：5

共 14 条

[1] ANALYSIS OF THE MONOENOIC FATTY-ACID DISTRIBUTION IN HYDROGENATED VEGETABLE-OILS BY SILVER-ION HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].