Intein-mediated in vitro synthesis of lipidated Ras proteins

被引：26

作者：

Gottlieb, D

Grunwald, C

Nowak, C

Kuhlmann, J

Waldmann, H

机构：

[1] Max Planck Inst Mol Physiol, Dept Biol Struct, D-44227 Dortmund, Germany

[2] Univ Dortmund, Dept Biol Chem, D-44227 Dortmund, Germany

来源：

CHEMICAL COMMUNICATIONS | 2006年 / 03期

关键词：

D O I：

10.1039/b511736d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Fully functional lipid-modified Ras proteins suitable for the study of Ras-membrane interactions and embodying exclusively native amide bonds can be synthesized in preparative amounts by means of Expressed Protein Ligation.

引用

页码：260 / 262

页数：3

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