Synthesis of adenine and guanine nucleotides at the 'inosinic branch point' in lymphocytes of leukemia patients

被引：11

作者：

Carlucci, F ^{[1
]}

Tabucchi, A ^{[1
]}

Pagani, R ^{[1
]}

Marinello, E ^{[1
]}

机构：

[1] Univ Siena, Ist Biochim & Enzimol, I-53100 Siena, Italy

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE | 1999年 / 1454卷 / 01期

关键词：

purine nucleotide; B-cell chronic lymphocytic leukemia; nucleotide synthesis;

D O I：

10.1016/S0925-4439(99)00032-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The synthesis of purine nucleotides has been studied in human peripheral blood lymphocytes from healthy subjects and patients affected by B-cell chronic lymphocytic leukemia (B-CLL). The rate of the synthesis was measured by following the incorporation of C-14-formate into the nucleotides of lymphocyte suspensions. The whole sequence AMP --> ADP --> ATP was found reduced in B-CLL lymphocytes; in the case of guanylates only the last step of the sequence GMP --> GDP --> GTP was significantly lower in the same cells. From the analysis of these results, combined with previous data, we conclude that purine metabolism undergoes an imbalancement during CLL, which is partially compensated, and point out the importance of studying concomitantly purine metabolism and nucleic acid synthesis in leukemia cells. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：106 / 114

页数：9

共 15 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] Purine nucleotide metabolism: Specific aspects in chronic lymphocytic leukemia lymphocytes [J].