Hemolysate inhibits L-type Ca2+ channels in rat basilar smooth muscle cells

被引：16

作者：

Kim, CJ ^{[1
]}

Weir, B ^{[1
]}

Macdonald, RL ^{[1
]}

Marton, LS ^{[1
]}

Zhang, H ^{[1
]}

机构：

[1] UNIV CHICAGO,DEPT SURG,NEUROSURG SECT,CHICAGO,IL 60637

来源：

JOURNAL OF VASCULAR RESEARCH | 1996年 / 33卷 / 03期

关键词：

Ca2+ channels; hemolysate; cerebral smooth muscle cells; vasospasm;

D O I：

10.1159/000159153

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Erythrocyte lysate increases intracellular Ca2+ ([Ca2+](i)) and contracts cerebral arteries in vitro and has been suggested to be the cause for cerebral vasospasm. We investigated the effect of hemolysate on L-type Ca2+ channels directly by using patch clamp techniques in freshly isolated single smooth muscle cells from rat basilar artery. Patch clamp studies revealed a whole-cell current which resembles the L-type Ca2+ current reported by others, Hemolysate reduced the amplitude of the L-type Ca2+ channel current. The effect of hemolysate was reversible by washout and repeatable. Hemolysate was separated into two fractions by using filter membranes. The fraction >1 kDa which contains oxyhemoglobin and other proteins mimicked the effect of hemolysate while the fraction <1 kDa and ATP were without effect. We conclude that hemolysate does not increase [Ca2+](i) by activation of L-type Ca2+ channel.

引用

收藏

页码：258 / 264

页数：7

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