A novel method for in vitro culture of plants: Cultivation of barley in a floating hydroponic system

被引:16
作者
Battke, F
Schramel, P
Ernst, D [1 ]
机构
[1] GSF, Inst Biochem Plant Pathol, Natl Res Ctr Environm & Hlth, D-85764 Neuherberg, Germany
[2] TUM, Lehrstuhl Pflanzenbau & Pflanzenzuchtung, D-85350 Freising Weihenstephan, Germany
[3] GSF, Inst Ecol Chem, Natl Res Ctr Environm & Hlth, D-85764 Neuherberg, Germany
关键词
Hordeum vulgare; heavy metal ions; hydroponics; polyethylene granulate;
D O I
10.1007/BF02772589
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We describe a system for hydroponically growing plants that is based on using a polyethylene (PE) granulate as the floating body. The procedure can be performed in all suitable vessels (e.g., bowls, basins, glasses) and is independent of the culture area. Barley plants (Hordeum vulgare) grown in this hydroponic system had no difference in the uptake of mercuric and cadmium ions when compared with plants grown on a defined agar medium. The simple and inexpensive method can be used for the isolation of leaf tissue and large amounts of root tissue. In addition, numerous experiments can be run in parallel.
引用
收藏
页码:405 / 409
页数:5
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