Enzymatic synthesis of 4-O-beta-D-galactopyranosyl-moranoline and 3-O-beta-D-galactopyranosyl-moranoline by using beta-galactosidase from Bacillus circulans

被引：7

作者：

Kojima, M

Seto, T

Kyotani, Y

Ogawa, H

Kitazawa, S

Mori, K

Maruo, S

Ohgi, T

Ezure, Y

机构：

[1] Discovery Research Lab III, Nippon Shinyaku Co Ltd, Kyoto, 601, Nishioji-hachijo, Minami-ku

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1996年 / 60卷 / 04期

关键词：

moranoline; 1-deoxynojirimycin; 3-O-beta-D-galactopyranosyl-moranoline; 4-O-beta-D-galactopyranosyl-moranoline; galactosidase;

D O I：

10.1271/bbb.60.694

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A transgalactosylation reaction from lactose to moranoline (1-deoxynojirimycin) was accomplished by using beta-galactosidase [EC 3.2.1.23] from Bacillus circulans. The enzyme formed 3-O-beta-D-galactopyranosyl-moranoline and 4-O-beta-D-galactopyransoyl-moranoline as major products, together with 2-O-beta-D-galactopyranosyl-moranoline and 6-O-beta-D-galactopyranosyl-moranoline as minor ones.

引用

页码：694 / 696

页数：3

共 10 条

[1] SIALYL LEWIS(X)-CONTAINING OLIGOSACCHARIDE ATTENUATES MYOCARDIAL REPERFUSION INJURY IN CATS [J].