An intron in the YRA1 gene is required to control Yra1 protein expression and mRNA export in yeast

被引：23

作者：

Rodríguez-Navarro, S ^{[1
]}

Strässer, K ^{[1
]}

Hurt, E ^{[1
]}

机构：

[1] BZH, D-69120 Heidelberg, Germany

来源：

EMBO REPORTS | 2002年 / 3卷 / 05期

关键词：

D O I：

10.1093/embo-reports/kvf091

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Yra1p is an essential and conserved mRNA export factor in yeast. Strikingly, removal of the intron from YRA1 causes a dominant-negative growth phenotype and a concomitant inhibition of mRNA export. However, both defects are neutralized by replacement of the intron of YRA1 by a different intron. Significantly, Yra1p is overproduced in yeast when expressed from its intronless gene, but Yra1p levels are the same as the wild type when expressed from an intron-containing YRA1 gene. Thus, an intron in YRA1 controls Yra1p expression and mRNA export.

引用

收藏

页码：438 / 442

页数：5

相关论文

共 33 条

[1] The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates [J].

Bachi, A ;

Braun, IC ;

Rodrigues, JP ;

Panté, N ;

Ribbeck, K ;

Von Kobbe, C ;

Kutay, U ;

Wilm, M ;

Görlich, D ;

Carmo-Fonseca, M ;

Izaurralde, E .

RNA, 2000, 6 (01) :136-158

[2]

Bear J, 1999, MOL CELL BIOL, V19, P6306

[3] TAP binds to the constitutive transport element (CTE) through a novel RNA-binding motif that is sufficient to promote CTE-dependent RNA export from the nucleus [J].

Braun, IC ;

Rohrbach, E ;

Schmitt, C ;

Izaurralde, E .

EMBO JOURNAL, 1999, 18 (07) :1953-1965

[4] ALY, a context-dependent coactivator of LEF-1 and AML-1, is required for TCR alpha enhancer function [J].

Bruhn, L ;

Munnerlyn, A ;

Grosschedl, R .

GENES & DEVELOPMENT, 1997, 11 (05) :640-653

[5] AN EFFICIENT METHOD TO ISOLATE YEAST GENES CAUSING OVEREXPRESSION-MEDIATED GROWTH ARREST [J].

ESPINET, C ;

DELATORRE, MA ;

ALDEA, M ;

HERRERO, E .

YEAST, 1995, 11 (01) :25-32

[6] PSEUDOGENES IN YEAST [J].

FINK, GR .

CELL, 1987, 49 (01) :5-6

[7] U2AF(65) recruits a novel human DEAD box protein required for the U2 snRNP-branchpoint interaction [J].

Fleckner, J ;

Zhang, M ;

Valcarcel, J ;

Green, MR .

GENES & DEVELOPMENT, 1997, 11 (14) :1864-1872

[8] The DExH/D box protein HEL/UAP56 is essential for mRNA nuclear export in Drosophila [J].

Gatfield, D ;

Le Hir, H ;

Schmitt, C ;

Braun, IC ;

Köcher, T ;

Wilm, M ;

Izaurralde, E .

CURRENT BIOLOGY, 2001, 11 (21) :1716-1721

[9] YEAST PROMOTERS - POSITIVE AND NEGATIVE ELEMENTS [J].

GUARENTE, L .

CELL, 1984, 36 (04) :799-800

[10] The DECD box putative ATPase Sub2p is an early mRNA export factor [J].

Jensen, TH ;

Boulay, J ;

Rosbash, M ;

Libri, D .

CURRENT BIOLOGY, 2001, 11 (21) :1711-1715

← 1 2 3 4 →