Intrinsic erythrocyte labeling and attomole pharmacokinetic tracing of 14C-labeled folic acid with accelerator mass spectrometry

被引:43
作者
Buchholz, BA
Arjomand, A
Dueker, SR
Schneider, PD
Clifford, AJ
Vogel, JS
机构
[1] Lawrence Livermore Natl Lab, Ctr Accelerator Mass Spectrometry, Livermore, CA 94551 USA
[2] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA
[3] Univ Calif Davis, Davis Canc Ctr, Sacramento, CA 95817 USA
关键词
intrinsic labeling; accelerator mass spectrometry; AMS; pharmacokinetics; folate; physiologic dose;
D O I
10.1006/abio.1999.4041
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Long-term physiologic tracing of nutrients, toxins, and drugs in healthy subjects is not possible using traditional decay counting of radioisotopes or stable isotope mass spectrometry due to radiation exposure and limited sensitivity, respectively. A physiologic dose of C-14-labeled folic acid (35 mu g, 100 nCi) was ingested by a healthy adult male and followed for 202 days in plasma, erythrocytes, urine, and feces using accelerator mass spectrometry. All samples and generated wastes were classified nonradioactive and the subject received a lifetime-integrated radiological effective dose of only II mu Sv. Radiolabeled folate appeared in plasma 10 min after ingestion but did not appear in erythrocytes until 5 days later. Approximately 0.4% of the erythrocytes were intrinsically labeled with an average of 130 C-14 atoms during erythropoiesis from the pulse of plasma [C-14]folate. An appropriate radiocarbon-labeled precursor can intrinsically label DNA or a specific protein during synthesis and obtain limits of quantitation several orders of magnitude below that of stable isotope methods. (C) 1999 Academic Press.
引用
收藏
页码:348 / 352
页数:5
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