Characterization of upper respiratory disease in rats following neonatal inoculation with a rat-adapted influenza virus

被引:8
作者
Dye, JA
Morgan, KT
Neldon, DL
Tepper, JS
Burleson, GR
Costa, DL
机构
[1] CIIT, RES TRIANGLE PK, NC USA
[2] UNIV N CAROLINA, CTR ENVIRONM MED & LUNG BIOL, CHAPEL HILL, NC USA
[3] MANTECH ENVIRONM TECHNOL INC, RES TRIANGLE PK, NC 27709 USA
关键词
influenza virus; nasal cavity; neonatal rats;
D O I
10.1177/030098589603300105
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Neonatal F344 rats were infected with a rat-adapted influenza virus (RAIV) to use as a potential model to study the combined effects of air pollutant exposure with early life respiratory viral infections. Initially, 6-day-old pups were intranasally inoculated with RAIV or medium alone, and nasal and lower respiratory tract (LRT) tissues were assessed histologically at 1, 3, 6, and 13 days postinoculation (DPI). Immunologic assessments included thymic lymphocyte quantification and anti-RAIV immunoglobulin production. Pups then received two inoculations (at 6 and 30 days of age), with histologic and immunologic assessment 6 and 13 days after the second inoculation and bronchoprovocation testing 5-8 weeks later. Following the single RAIV inoculation, IgM and IgG(1) measurements increased at 6, 11, and 15 DPI, with IgG(1) being greater at 11 and 15 DPI. Nasal lesions were evident as early as 1 DPI and primarily involved the anterior dorsal medial meatus and adjacent dorsal atrio- and nasoturbinates. Alterations included epithelial cell exfoliation and necrosis, mild erosions, suppurative and nonsuppurative inflammation, intraepithelial neutrophil accumulations, and intraluminal exudate. By 3 DPI, olfactory epithelial damage was multifocal or locally diffuse, with degeneration of sensory cells and variable inflammation. By 13 DPI, lesions were essentially repaired. Minimal changes were apparent in the LRT despite evidence of viral replication in the lungs 24 hours after inoculation (>3 log(10) plaque-forming units/lung). Pups reinoculated with RAIV at 30 days of age did not develop significant histologic lesions, nor did they exhibit increased airway responsiveness when assessed as young adults. In spite of their immature immune status at the time of initial infection, 13 days after the second RAIV inoculation, IgG(1) increased substantially. Thus, neonatal RAIV infection resulted in acute nasal epithelial injury and inflammation, alterations that may allow subsequent evaluation of viral disease-air pollutant interactions.
引用
收藏
页码:43 / 54
页数:12
相关论文
共 44 条
[1]   AIR-POLLUTION AND HOSPITAL ADMISSIONS IN SOUTHERN ONTARIO - THE ACID SUMMER HAZE EFFECT [J].
BATES, DV ;
SIZTO, R .
ENVIRONMENTAL RESEARCH, 1987, 43 (02) :317-331
[2]   NEUTRAL ENDOPEPTIDASE AND NEUROGENIC INFLAMMATION IN RATS WITH RESPIRATORY-INFECTIONS [J].
BORSON, DB ;
BROKAW, JJ ;
SEKIZAWA, K ;
MCDONALD, DM ;
NADEL, JA .
JOURNAL OF APPLIED PHYSIOLOGY, 1989, 66 (06) :2653-2658
[3]  
BROWN HR, 1989, TOXICOLOGIST, V9, P37
[4]  
BRUNET G, 1983, J IMMUNOL, V131, P434
[5]  
CASTLEMAN WL, 1990, AM J PATHOL, V137, P821
[6]  
CASTLEMAN WL, 1988, LAB INVEST, V59, P387
[7]  
DOCKERY DW, 1993, AM REV RESPIR DIS, V147, pA633
[8]   VIRUS INDUCES AIRWAY HYPERRESPONSIVENESS TO TACHYKININS - ROLE OF NEUTRAL ENDOPEPTIDASE [J].
DUSSER, DJ ;
JACOBY, DB ;
DJOKIC, TD ;
RUBINSTEIN, I ;
BORSON, DB ;
NADEL, JA .
JOURNAL OF APPLIED PHYSIOLOGY, 1989, 67 (04) :1504-1511
[9]  
Ehrlich J. P., 1989, INHAL TOXICOL, V1, P129
[10]  
EHRLICH JP, 1991, THESIS NEW YORK U NE