A role for transcriptional repressors in targeting the yeast Swi/Snf complex

被引：92

作者：

Dimova, D

Nackerdien, Z

Furgeson, S

Eguchi, S

Osley, MA ^{[1
]}

机构：

[1] Mem Sloan Kettering Canc Ctr, Program Mol Biol, New York, NY 10021 USA

[2] Cornell Univ, Grad Sch Med Sci, New York, NY 10021 USA

来源：

MOLECULAR CELL | 1999年 / 4卷 / 01期

关键词：

D O I：

10.1016/S1097-2765(00)80189-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genetic and biochemical studies indicate that the evolutionarily conserved Swi/Snf complex acts at a subset of genes to help transcriptional activators function on chromatin templates. The mechanism by which this complex is targeted to specific chromosomal loci remains unknown. We show that Swi/Snf is required for expression of the yeast histone HTA1-HTB1 locus because of the role of Hir1p and Hir2p corepressors in negatively regulating transcription. Snf5p, Snf2p/Swi2p, and Swi3p, three components of the yeast Swi/Snf complex, coimmunoprecipitate with each Hir protein, and Snf5p is maximally associated with the HTA1-HTB1 promoter when the Hir-based repression system is intact and the Swi/Snf complex is functional. The data support a role for the Hir repressors in the gene-specific targeting of Swi/Snf.

引用

页码：75 / 83

页数：9

共 83 条

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