Dehydrogenation polymer-cell wall complexes as a model for lignified grass walls

被引：51

作者：

Grabber, JH ^{[1
]}

Ralph, J ^{[1
]}

Hatfield, RD ^{[1
]}

Quideau, S ^{[1
]}

Kuster, T ^{[1
]}

Pell, AN ^{[1
]}

机构：

[1] CORNELL UNIV, DEPT ANIM SCI, ITHACA, NY 14853 USA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1996年 / 44卷 / 06期

关键词：

gramineae; Zea mays; cell wall; fiber; peroxidase; p-hydroxycinnamyl alcohols; lignin; dehydrogenation polymer; transmission electron; microscopy; pyrolysis; thioacidolysis; NMR; fiber degradability;

D O I：

10.1021/jf9502717

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

p-Hydroxycinnamyl alcohols were efficiently polymerized into maize walls by wall-bound peroxidases and in vitro generated hydrogen peroxide to produce dehydrogenation polymer-cell wall (DHP-CW) complexes. Electron microscopy of KMnO4-stained sections revealed that DHPs were distributed throughout the cell wall matrix. DHP-CW complexes were structurally similar to natural grass lignins according to pyrolysis, thioacidolysis, and C-13-NMR spectroscopy. Nonlignified walls were rapidly degraded by rumen microorganisms and by commercial fungal hydrolases, whereas DHP-CW complexes had a reduced rate and extent of degradation. This system is useful for modeling matrix interactions in lignified walls and for identifying means of improving the utilization of lignocellulosic materials for nutritional and industrial purposes.

引用

页码：1453 / 1459

页数：7

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