An investigation into the immunogenicity of a GnRH analogue in male rats: A comparison of the toxicity of various adjuvants used in conjunction with GnRH-glycys

Immunization of male Copenhagen Fischer vats with a gonadotrophin releasing hormone (GnRH) analogue, conjugated to PPD resulted in hi,oh levels of antibody being produced which disrupted gonadal function in male rats. The antibody reduced serum testosterone levels and subsequently suppressed spermatogenesis. Alternatives to Freund's adjuvant were tested namely, aluminium hydroxide and non-ionic surfactant vesicles (NISV). The study showed that aluminium hydroxide was as effective as Freund's adjuvant and less toxic, in both BCG and non-BCG primed animals. However, NISV were completely non-toxic and most effective in conjunction with BCG priming. The data obtained showed that NISV have the potential to be wed as an alternative to FCA and aluminium hydroxide-based adjuvants. Copyright (C) 1996 Published by Elsevier Science Ltd.