Production in Escherichia coli of moricin, a novel type antibacterial peptide from the silkworm, Bombyx mori

被引:56
作者
Hara, S
Yamakawa, M
机构
[1] NATL INST SERICULTURAL & ENTOMOL SCI,LAB BIOL DEF,TSUKUBA,IBARAKI 305,JAPAN
[2] NODA INST SCI RES,NODA,CHIBA 278,JAPAN
关键词
D O I
10.1006/bbrc.1996.0461
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Moricin is a novel type antibacterial peptide recently isolated from the silkworm, Bombyx mori. Two foreign gene expression systems in Escherichia coli were employed to obtain a large amount of the peptide for further characterization. An artificial moricin gene was chemically synthesized and inserted into two expression vectors, pXa1 and pMAL-c2. The recombinant moricin was efficiently produced in E. coli as fusion proteins and released by chemical cleavage with cyanogen bromide or o-iodosobenzoic acid. Eleven milligrams of pure recombinant moricin was obtained from 2 L of E. coli culture. The primary structure and molecular mass of the purified recombinant moricin was the same as those of the natural moricin. In addition, the antibacterial activity of the recombinant moricin against E. coli and Staphylococcus aureus was comparable to that of the natural moricin. (C) 1996 Academic Press, Inc.
引用
收藏
页码:664 / 669
页数:6
相关论文
共 20 条
[1]  
BOMAN HG, 1994, CIBA F S, V186
[2]  
BURK RR, 1983, METHOD ENZYMOL, V91, P247
[3]  
FONTANA A, 1983, METHOD ENZYMOL, V91, P311
[4]  
Gross E., 1967, Methods Enzymol, V11, P238, DOI [10.1016/S0076-6879(67)11029-X, DOI 10.1016/S0076-6879(67)11029-X]
[5]  
HARA S, 1995, J BIOL CHEM, V270, P29923, DOI 10.1074/jbc.270.50.29923
[6]   INSECT IMMUNITY - ISOLATION AND STRUCTURE OF CECROPIN-D AND 4 MINOR ANTIBACTERIAL COMPONENTS FROM CECROPIA PUPAE [J].
HULTMARK, D ;
ENGSTROM, A ;
BENNICH, H ;
KAPUR, R ;
BOMAN, HG .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1982, 127 (01) :207-217
[7]   SYNTHESIS OF A GENE FOR HUMAN GROWTH-HORMONE AND ITS EXPRESSION IN ESCHERICHIA-COLI [J].
IKEHARA, M ;
OHTSUKA, E ;
TOKUNAGA, T ;
TANIYAMA, Y ;
IWAI, S ;
KITANO, K ;
MIYAMOTO, S ;
OHGI, T ;
SAKURAGAWA, Y ;
FUJIYAMA, K ;
IKARI, T ;
KOBAYASHI, M ;
MIYAKE, T ;
SHIBAHARA, S ;
ONO, A ;
UEDA, T ;
TANAKA, T ;
BABA, H ;
MIKI, T ;
SAKURAI, A ;
OISHI, T ;
CHISAKA, O ;
MATSUBARA, K .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (19) :5956-5960
[9]   EXPRESSION IN ESCHERICHIA-COLI OF A CHEMICALLY SYNTHESIZED GENE FOR HORMONE SOMATOSTATIN [J].
ITAKURA, K ;
HIROSE, T ;
CREA, R ;
RIGGS, AD ;
HEYNEKER, HL ;
BOLIVAR, F ;
BOYER, HW .
SCIENCE, 1977, 198 (4321) :1056-1063
[10]   FRAGMENTATION OF PROTEINS WITH ORTHO-IODOSOBENZOIC ACID - CHEMICAL MECHANISM AND IDENTIFICATION OF ORTHO-IODOXYBENZOIC ACID AS A REACTIVE CONTAMINANT THAT MODIFIES TYROSYL RESIDUES [J].
MAHONEY, WC ;
SMITH, PK ;
HERMODSON, MA .
BIOCHEMISTRY, 1981, 20 (02) :443-448