Ligand exchange capillary electrophoresis by cyclodextrin derivatives, a powerful tool for enantiorneric separations

被引：27

作者：

Cucinotta, Vincenzo ^{[1
]}

Giuffrida, Alessandro ^{[1
]}

Maccarrone, Giuseppe ^{[1
]}

Messina, Marianna ^{[1
]}

Vecchio, Graziella ^{[1
]}

机构：

[1] Univ Catania, Dept Chem Sci, I-95125 Catania, Italy

来源：

ELECTROPHORESIS | 2006年 / 27卷 / 08期

关键词：

amino acid; chiral separation; cyclodextrin; ligand exchange CE; stereoselectivity;

D O I：

10.1002/elps.200500755

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Five pure CD derivatives synthesized in our laboratory were used as chiral selectors in the presence of copper(II) ion. Three enantiomeric pairs of amino acids were submitted to separation experiments in CE, by exploiting the ligand exchange mechanism. The results obtained in the investigated systems, together with those of the analogous systems previously studied, clearly show the usefulness of this technique in chiral separations. By comparing the ligand exchange CE results with potentiometric results, either reported elsewhere or studied here for the first time (system Cu/CDampy/tyrosine), it has been possible to rationalise the separation results. The importance of the availability of pure selectors, and to characterise them both spectroscopically and thermodynamically is discussed.

引用

页码：1471 / 1480

页数：10

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[1] NONLINEAR LEAST-SQUARES APPROACH TO THE REFINEMENT OF ALL PARAMETERS INVOLVED IN ACID-BASE TITRATIONS [J].