Roles of Molecular Layer Interneurons in Sensory Information Processing in Mouse Cerebellar Cortex Crus II In Vivo

被引:42
作者
Chu, Chun-Ping [1 ,3 ]
Bing, Yan-Hua [1 ,2 ]
Liu, Heng [1 ,2 ]
Qiu, De-Lai [1 ,2 ]
机构
[1] Yanbian Univ, Cellular Funct Res Ctr, Yanji, Jilin Province, Peoples R China
[2] Yanbian Univ, Coll Med, Dept Physiol & Pathophysiol, Yanji, Jilin Province, Peoples R China
[3] Yanbian Univ, Coll Basic Med, Funct Expt Ctr, Yanji, Jilin Province, Peoples R China
基金
中国国家自然科学基金;
关键词
FIBER RECEPTIVE-FIELDS; PURKINJE-CELLS; GRANULE CELL; SYNCHRONIZED ACTIVITY; SYNAPTIC CURRENTS; GABA(A) RECEPTORS; BASKET CELLS; PARALLEL; INHIBITION; RAT;
D O I
10.1371/journal.pone.0037031
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];
摘要
Background: Cerebellar cortical molecular layer interneurons (MLIs) play essential roles in sensory information processing by the cerebellar cortex. However, recent experimental and modeling results are questioning traditional roles for molecular layer inhibition in the cerebellum. Methods and Main Results: Synaptic responses of MLIs and Purkinje cells (PCs), evoked by air-puff stimulation of the ipsilateral whisker pad were recorded from cerebellar cortex Crus II in urethane-anesthetized ICR mice by in vivo whole-cell patch-clamp recording techniques. Under current-clamp (I = 0), air-puff stimuli were found to primarily produce inhibition in PCs. In MLIs, this stimulus evoked spike firing regardless of whether they made basket-type synaptic connections or not. However, MLIs not making basket-type synaptic connections had higher rates of background activity and also generated spontaneous spike-lets. Under voltage-clamp conditions, excitatory postsynaptic currents (EPSCs) were recorded in MLIs, although the predominant response of recorded PCs was an inhibitory postsynaptic potential (IPSP). The latencies of EPSCs were similar for all MLIs, but the time course and amplitude of EPSCs varied with depth in the molecular layer. The highest amplitude, shortest duration EPSCs were recorded from MLIs deep in the molecular layer, which also made basket-type synaptic connections. Comparing MLI to PC responses, time to peak of PC IPSP was significantly slower than MLI recorded EPSCs. Blocking GABA(A) receptors uncovered larger EPSCs in PCs whose time to peak, half-width and 10-90% rising time were also significantly slower than in MLIs. Biocytin labeling indicated that the MLIs (but not PCs) are dye-coupled. Conclusions: These findings indicate that tactile face stimulation evokes rapid excitation in MLIs and inhibition occurring at later latencies in PCs in mouse cerebellar cortex Crus II. These results support previous suggestions that the lack of parallel fiber driven PC activity is due to the effect of MLI inhibition.
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页数:8
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