Mediating phosphorylation events in the vanadium-induced respiratory burst of alveolar macrophages

被引:32
作者
Grabowski, GM [1 ]
Paulauskis, JD [1 ]
Godleski, JJ [1 ]
机构
[1] Harvard Univ, Sch Publ Hlth, Dept Environm Hlth, Boston, MA 02115 USA
关键词
alveolar macrophages; epidermal growth factor receptor; hydrogen peroxide; inflammation; NADPH oxidase; respiratory burst; reactive oxygen intermediates; sodium metavanadate; tyrosine phosphorylation; vanadyl chloride;
D O I
10.1006/taap.1999.8642
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Occupational exposure by inhalation to vanadium-containing particles such as residual oil fly ash results in respiratory tract inflammation. This inflammation, characterized by abundant neutrophilia, appears to be initiated by alveolar macrophages (AMs) encountering particles and the subsequent release of proinflammatory cytokines. Intracellular signaling events in these cells in response to particles or their components are largely unknown. We investigated two immediate responses of AMs to vanadium exposure in vitro, the production of reactive oxygen intermediates (ROI) or respiratory burst (RB), and the tyrosine phosphorylation of cellular proteins. Macrophages exposed in vitro to 100 mu M vanadyl chloride/1 mu Gi V-48 incorporated 8.3% of the metal after 30 min. Exposure of AMs to increasing concentrations of sodium metavanadate resulted in a dose-dependent increase in production of ROI as measured by dichlorofluorescin oxidation. The lowest dose yielding a significant response was 50 mu M, whereas 1000 mu M increased RE activity by 173%. NADPH oxidase inhibitors deoxy-D-glucose (100 mM) and diphenylene iodonium (25 mu M) reduced the metavanadate-induced RE by 62 and 71%, respectively, implicating NADPH oxidase as the primary cellular source of ROI. Enhanced cerium chloride oxidation in response to metavanadate localized to the plasma membrane consistent with increased NADPH oxidase activity. Pretreatment of AMs with the epidermal growth factor receptor inhibitor, tryphostin B50 (10 mu M), reduced the metavanadate-induced RE, but did not influence overall tyrosine phosphorylation, Metavanadate and H2O2 exposure greatly increased overall tyrosine phosphorylation, yielding a similar but distinguishable pattern of phosphorylation in these cells. These observations demonstrate that in vitro metavanadate exposure regulates two distinct, yet related intracellular signaling pathways important in initiating inflammatory responses in these cells: (1) activation of the NADPH oxidase complex with subsequent increased ROI synthesis, and (2) enhanced tyrosine phosphorylation of cellular proteins. (C) 1999 Academic Press.
引用
收藏
页码:170 / 178
页数:9
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