Rapid isolation of yeast plasmids as native chromatin

被引：3

作者：

MartinezCampa, C ^{[1
]}

Kent, NA ^{[1
]}

Mellor, J ^{[1
]}

机构：

[1] UNIV OXFORD,DEPT BIOCHEM,MICROBIOL UNIT,OXFORD OX1 3QU,ENGLAND

来源：

NUCLEIC ACIDS RESEARCH | 1997年 / 25卷 / 09期

基金：

英国惠康基金;

关键词：

D O I：

10.1093/nar/25.9.1872

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Many regions of chromatin are subject to dynamic changes. We have developed a rapid method for isolation of small chromatin templates from yeast which will facilitate biochemical analysis of chromatin composition. Using the PHO5 promoter we show that templates prepared from cells grown in inducing or repressing conditions show native chromatin structures. This method may be widely applicable as the chromatin structures at a centromere, at ARS1 and at part of the lacZ region on two other plasmids are preserved after chromatin isolation.

引用

页码：1872 / 1873

页数：2

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