Ultratrace analysis for trans,trans-muconic acid by electrophoric derivatization and capillary gas chromatography

被引：5

作者：

Lin, FM ^{[1
]}

Wu, HL ^{[1
]}

Kou, HS ^{[1
]}

Lin, SJ ^{[1
]}

机构：

[1] Kaohsiung Med Univ, Grad Inst Pharmaceut Sci, Kaohsiung 807, Taiwan

来源：

ANALYTICA CHIMICA ACTA | 2002年 / 455卷 / 01期

关键词：

trans; trans-muconic acid; benzene biomarker; derivatization; GC-ECD;

D O I：

10.1016/S0003-2670(01)01573-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A highly sensitive method is described for the determination of trans, trans-muconic acid (MA), a biomarker of benzene exposure. The method is based on the derivatization of MA with an electrophoric reagent. 2-(pentafluorophenoxy)ethyl-2-(piperidino)ethanesulfonate, using potassium carbonate and 18-crown-6 ether as reaction activators, The resulting pentafluorophenoxy derivative of MA was analyzed by capillary GC with an electron-capture detector (ECD). The lower quantitation limit of the method is attainable at 0.3 muM of MA with a detection limit of about 60 nM (S/N = 3) (60 fmol per 1.0 mul injection). Application of the method to the analysis of MA in urine proved feasible. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：111 / 116

页数：6

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