Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells

被引：117

作者：

Owen, Dylan M. ^{[1
]}

Lanigan, Peter M. P.

Dunsby, Christopher

Munro, Ian

Grant, David

Neil, Mark A. A.

French, Paul M. W.

Magee, Anthony I.

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Chem Biol Ctr, London, England

[2] Univ London Imperial Coll Sci Technol & Med, Dept Phys, London, England

[3] Univ London Imperial Coll Sci Technol & Med, Div Biomed Sci, London, England

来源：

BIOPHYSICAL JOURNAL | 2006年 / 90卷 / 11期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1529/biophysj.106.084673

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We apply fluorescence lifetime imaging to the membrane phase-sensing dye di-4-ANEPPDHQ in model membranes and live cells. We show that the 1700 ps lifetime shift between liquid-disordered and liquid-ordered phases offers greater contrast than the 60 nm spectral shift previously reported. Detection of cholesterol-rich membrane microdomains is confirmed by observation of the temperature dependence of membrane order and by cholesterol depletion using methyl-beta-cyclodextrin.

引用

页码：L80 / L82

页数：3

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