Uncoated, broad fluorescent, and size-homogeneous CdSe quantum dots for bioanalyses

被引：73

作者：

Zhelev, Z

Bakalova, R

Ohba, H

Jose, R

Imai, Y

Baba, Y

机构：

[1] AIST, Onsite Sensing & Diagnost Res Lab, Tosu, Saga, Japan

[2] Toyota Technol Inst, Nagoya, Aichi 468, Japan

[3] AIST, Technol Res Ctr, Takamatsu, Kagawa, Japan

[4] Nagoya Univ, Grad Sch Engn, Dept Appl Chem, Nagoya, Aichi, Japan

来源：

ANALYTICAL CHEMISTRY | 2006年 / 78卷 / 01期

关键词：

D O I：

10.1021/ac0511896

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In the present study, we describe the synthesis of highly luminescent uncoated water-soluble CdSe quantum dots (QDs) possessing the following characteristics: similar to 2 nm in diameter, with very good size distribution (in 95% homodispersed) accompanied by a broad-band photoluminescent spectrum. The synthetic procedure is simple, is conducted at room temperature, in the absence of the most popular coordinating ligands (as TOPO or HDA), and is highly reproducible. The obtained CdSe core QDs possessed a comparatively long fluorescence half-life (similar to 30-90 ns, depending on the emission wavelength) detected by time-resolved spectroscopy. These QDs were further conjugated with antibodies and applied in several biochemical analyses.

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页码：321 / 330

页数：10

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