The surprising complexity of the transcriptional regulation of the spdri gene reveals the existence of new linkages inside sea urchin's PMC and Oral Ectoderm Gene Regulatory Networks

被引:1
作者
Al Mahmud, Abdullah [1 ]
Amore, Gabriele [1 ]
机构
[1] Stn Zool Anton Dohrn, Mol Evolut Grp, I-80121 Naples, Italy
关键词
Spdeadringer; spdri; Sea urchin embryo; cis-regulation; Endomesoderm; PMCs; Oral Ectoderm; Gene Regulatory Networks;
D O I
10.1016/j.ydbio.2008.07.036
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
During sea urchin embryogenesis the spdri gene participates in two separate Gene Regulatory Networks (GRNs): the Primary Mesenchyme Cells'(PMCs) and the Oral Ectoderm's one. In both cases, activation of the gene follows initial specification events [Amore, G., Yavrouian, R., Peterson, K., Ransick, A., McClay, D., Davidson, E., 2003. Spdeadringer, a sea urchin embryo gene required separately in skeletogenic and oral ectoderm gene regulatory networks. Dev. Biol. 261, 55-81.]. We identified a portion of genomic DNA ("4.7IL" - 3456;+389) which is sufficient to replicate sdpri's expression pattern in experiments of transgenesis, using a GFP reporter. In our experiments, the activation kinetic of 4.7IL-GFP was similar to that of the endogenous gene and the reporter responded to known spdri's transcriptional regulators (Ets1, Alx1, Gsc and Dri). Here we present a dissection of this regulatory region and a description of the modules involved in spdri's transcriptional regulation. Both in the PMCs' and Oral Ectoderm's expression phases, activation of spdri is obtained through the integration of three kinds of inputs: positive and globally distributed ones; negative ones (that prevent ectopic expression): positive and tissue-specific ones. Our results allow to expand the map of the regulatory connections at the spdri node, both in the PMCs and in the Oral Ectoderm Gene Regulatory Networks (GRNs). (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码:425 / 434
页数:10
相关论文
共 37 条
[1]   Spdeadringer, a sea urchin embryo gene required separately in skeletogenic and oral ectoderm gene regulatory networks [J].
Amore, G ;
Yavrouian, RG ;
Peterson, KJ ;
Ransick, A ;
McClay, DR ;
Davidson, EH .
DEVELOPMENTAL BIOLOGY, 2003, 261 (01) :55-81
[2]   cis-Regulatory control of cyclophilin, a member of the ETS-DRI skeletogenic gene battery in the sea urchin embryo [J].
Amore, Gabriele ;
Davidson, Enic H. .
DEVELOPMENTAL BIOLOGY, 2006, 293 (02) :555-564
[3]  
Angerer LM, 2001, DEVELOPMENT, V128, P4393
[4]   p38 MAPK is essential for secondary axis specification and patterning in sea urchin embryos [J].
Bradham, CA ;
McClay, DR .
DEVELOPMENT, 2006, 133 (01) :21-32
[5]   New computational approaches for analysis of cis-regulatory networks [J].
Brown, CT ;
Rust, AG ;
Clarke, PJC ;
Pan, Z ;
Schilstra, MJ ;
De Buysscher, T ;
Griffin, G ;
Wold, BJ ;
Cameron, RA ;
Davidson, EH ;
Bolouri, H .
DEVELOPMENTAL BIOLOGY, 2002, 246 (01) :86-102
[6]   Human CART1, a paired-class homeodomain protein, activities transcription through palindromic binding sites [J].
Cai, RL .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 250 (02) :305-311
[7]   cis-regulatory activity of randomly chosen genomic fragments from the sea urchin [J].
Cameron, RA ;
Oliveri, P ;
Wyllie, J ;
Davidson, EH .
GENE EXPRESSION PATTERNS, 2004, 4 (02) :205-213
[8]   Oral-aboral axis specification in the sea urchin embryo -: II.: Mitochondrial distribution and redox state contribute to establishing polarity in Strongylocentrotus purpuratus [J].
Coffman, JA ;
McCarthy, JJ ;
Dickey-Sims, C ;
Robertson, AJ .
DEVELOPMENTAL BIOLOGY, 2004, 273 (01) :160-171
[9]   Mitochondria, redox signaling and axis specification in metazoan embryos [J].
Coffman, James A. ;
Denegre, James M. .
DEVELOPMENTAL BIOLOGY, 2007, 308 (02) :266-280
[10]   Functional characterization of Ets-binding sites in the sea urchin embryo: three base pair conversions redirect expression from mesoderm to ectoderm and endoderm [J].
Consales, C ;
Arnone, MI .
GENE, 2002, 287 (1-2) :75-81