Endogenous mast cell degranulation modulates cervical contractility in the guinea pig

被引:34
作者
Bytautiene, E
Vedernikov, YP
Saade, GR
Romero, R
Garfield, RE
机构
[1] Univ Texas, Med Branch, Dept Obstet & Gynecol, Div Reprod Sci, Galveston, TX 77555 USA
[2] NICHHD, Perinatol Res Branch, Bethesda, MD USA
关键词
mast cells; cervix; contractility; pregnancy; hypersensitivity;
D O I
10.1067/mob.2002.120488
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
OBJECTIVE: The purpose of this study was to investigate the effect of endogenous mast cell degranulation on the contractility of isolated cervical strips from nonpregnant and pregnant guinea pigs. STUDY DESIGN: Longitudinal cervical strips from nonpregnant and pregnant (mid and term) guinea pigs were used for isometric tension recording. Responses to the mast cell degranulating agent, compound 48/80, were compared in the absence or presence of different inhibitors and receptor antagonists. Concentration-response curves were obtained to histamine and 5-hydroxytryptamine in strips that were incubated with antagonists or solvent. RESULTS: Compound 48/80 and histamine significantly increased contractility of cervical strips in all 3 groups of animals. The inhibitor of mast cell degranulation significantly reduced responses to compound 48/80 and histamine-1 receptor antagonist reduced responses to histamine in all 3 groups. Histamine-1 receptor antagonist significantly inhibited responses to compound 48/80 in nonpregnant and mid pregnant guinea pigs. Histamine-2 receptor antagonist did not alter responses to compound 48/80 nor to histamine. The receptor antagonist 5-hydroxytryptamine-2 significantly inhibited cervical contractility that was induced by compound 48/80 in tissues from mid pregnant and term pregnant guinea pigs. Lipoxygenase inhibitor was effective in mid pregnant guinea pigs. Cyclooxygenase inhibitor, 5-hydroxytryptamine, and a combination of lipoxygenase and cyclooxygenase inhibitors had no effect on cervical contractility. CONCLUSION: The degranulation of mast cells releases histamine and other mediators that stimulate cervical contractility through histamine-1 receptors. Cervical infiltration and modulation of contractility by mast cells may play an important physiologic and/or pathologic role in the control of cervical function during pregnancy.
引用
收藏
页码:438 / 445
页数:8
相关论文
共 22 条
[1]  
BEFUS AD, 1982, J IMMUNOL, V128, P2475
[2]  
BRYMAN I, 1986, OBSTET GYNECOL, V67, P574
[3]  
BRYMAN I, 1985, ACTA PHYSIOL HUNG, V65, P327
[4]  
BRYMAN I, 1984, OBSTET GYNECOL, V63, P280
[5]  
BRYMAN I, 1990, OBSTET GYNECOL, V75, P240
[6]   CERVICAL RIPENING WITH THE CYTOKINES INTERLEUKIN-8, INTERLEUKIN-1-BETA AND TUMOR-NECROSIS-FACTOR-ALPHA IN GUINEA-PIGS [J].
CHWALISZ, K ;
BENSON, M ;
SCHOLZ, P ;
DAUM, J ;
BEIER, HM ;
HEGELEHARTUNG, C .
HUMAN REPRODUCTION, 1994, 9 (11) :2173-2181
[7]   EFFECTS OF HISTAMINE AND SEROTONIN ON THE CONTRACTILITY OF ISOLATED PREGNANT AND NONPREGNANT HUMAN MYOMETRIUM [J].
CRUZ, MA ;
GONZALEZ, C ;
ACEVEDO, CG ;
SEPULVEDA, WH ;
RUDOLPH, MI .
GYNECOLOGIC AND OBSTETRIC INVESTIGATION, 1989, 28 (01) :1-4
[8]  
EKEHOVD E, 1998, MOL HUM REPROD, V4, P915
[9]   Modulation of rat uterine contractility by mast cells and their mediators [J].
Garfield, RE ;
Bytautiene, E ;
Vedernikov, YP ;
Marshall, JS ;
Romero, R .
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 2000, 183 (01) :118-125
[10]   PHARMACOLOGICAL INVESTIGATIONS INTO THE EFFECTS OF HISTAMINE AND HISTAMINE ANALOGS ON GUINEA-PIG AND RAT UTERUS [J].
GOYAL, RK ;
VERMA, SC .
AGENTS AND ACTIONS, 1981, 11 (04) :312-317