Casein kinase 2 is essential for mitophagy

被引：131

作者：

Kanki, Tomotake ^{[1
]}

Kurihara, Yusuke ^{[1
,2
]}

Jin, Xiulian ^{[1
]}

Goda, Tadahiro ^{[2
]}

Ono, Yusuke ^{[3
]}

Aihara, Masamune ^{[2
]}

Hirota, Yuko ^{[1
]}

Saigusa, Tetsu ^{[1
]}

Aoki, Yoshimasa ^{[2
]}

Uchiumi, Takeshi ^{[2
]}

Kang, Dongchon ^{[2
]}

机构：

[1] Niigata Univ, Grad Sch Med & Dent Sci, Lab Biosignaling, Niigata 9518510, Japan

[2] Kyushu Univ, Dept Clin Chem & Lab Med, Grad Sch Med Sci, Fukuoka 8128582, Japan

[3] Kyushu Univ, Sch Med, Fukuoka 8128582, Japan

来源：

EMBO REPORTS | 2013年 / 14卷 / 09期

关键词：

autophagy; mitochondria; mitophagy; yeast; casein kinase 2; YEAST SACCHAROMYCES-CEREVISIAE; BETA-SUBUNIT; AUTOPHAGY; PROTEIN; CK2; PHOSPHORYLATION; MITOCHONDRIA; ATG32; MATURATION; P62/SQSTM1;

D O I：

10.1038/embor.2013.114

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Mitophagy is a process that selectively degrades mitochondria. When mitophagy is induced in yeast, the mitochondrial outer membrane protein Atg32 is phosphorylated, interacts with the adaptor protein Atg11 and is recruited into the vacuole with mitochondria. We screened kinase-deleted yeast strains and found that CK2 is essential for Atg32 phosphorylation, Atg32-Atg11 interaction and mitophagy. Inhibition of CK2 specifically blocks mitophagy, but not macroautophagy, pexophagy or the Cvt pathway. In vitro, CK2 phosphorylates Atg32 at serine 114 and serine 119. We conclude that CK2 regulates mitophagy by directly phosphorylating Atg32.

引用

页码：788 / 794

页数：7

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