SEPS1 gene is activated during astrocyte ischemia and shows prominent antiapoptotic effects

被引：52

作者：

Fradejas, Noelia ^{[1
]}

Pastor, Maria Dolores ^{[1
]}

Mora-Lee, Silvia ^{[1
]}

Tranque, Pedro ^{[1
]}

Calvo, Soledad ^{[1
]}

机构：

[1] Univ Castilla La Mancha, Fac Med, Dept Ciencias Med, Pharmacol Unit,Ctr Reg Invest Biomed, Albacete 02006, Spain

来源：

JOURNAL OF MOLECULAR NEUROSCIENCE | 2008年 / 35卷 / 03期

关键词：

selenoproteins; ER stress; apoptosis; OGD; glial cells;

D O I：

10.1007/s12031-008-9069-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Contrarily to neurons, astrocytes can survive short periods of ischemia. We have searched for genes implicated in astrocyte resistance to ischemia using oxygen and glucose deprivation (OGD) as a stroke model. A RNA differential display approach uncovered the OGD induction of selenoprotein-S-encoding gene SEPS1. This endoplasmic reticulum (ER) resident protein is known to promote cell survival regulating the ER stress as well as inflammation. We found that suppression of SEPS1 by small interfering RNA severely increases astrocyte injure caused by OGD, suggesting that selenoprotein S protects astrocytes against ischemia. Our data also support that modulation of ER stress is implicated in this effect.

引用

收藏

页码：259 / 265

页数：7

相关论文

共 28 条

[1]

Variation in the selenoprotein S gene locus is associated with coronary heart disease and ischemic stroke in two independent Finnish cohorts [J].

Alanne, Mervi ;

Kristiansson, Kati ;

Auro, Kirsi ;

Silander, Kaisa ;

Kuulasmaa, Kari ;

Peltonen, Leena ;

Salomaa, Veikko ;

Perola, Markus .

HUMAN GENETICS, 2007, 122 (3-4) :355-365

[2]

Selenium protects cerebral ischemia in rat brain mitochondria [J].

Ansari, MA ;

Ahmad, AS ;

Ahmad, M ;

Salim, S ;

Youscuf, S ;

Ishrat, T ;

Islam, F .

BIOLOGICAL TRACE ELEMENT RESEARCH, 2004, 101 (01) :73-86

[3]

CHOP plays a pivotal role in the astrocyte death induced by oxygen and glucose deprivation [J].

Benavides, A ;

Pastor, D ;

Santos, P ;

Tranque, P ;

Calvo, S .

GLIA, 2005, 52 (04) :261-275

[4]

Selenium and selenoproteins in the brain and brain diseases [J].

Chen, J ;

Berry, MJ .

JOURNAL OF NEUROCHEMISTRY, 2003, 86 (01) :1-12

[5]

Increased infarct size and exacerbated apoptosis in the glutathione peroxidase-1 (Gpx-1) knockout mouse brain in response to ischemia/reperfusion injury [J].

Crack, PJ ;

Taylor, JM ;

Flentjar, NJ ;

de Haan, J ;

Hertzog, P ;

Iannello, RC ;

Kola, I .

JOURNAL OF NEUROCHEMISTRY, 2001, 78 (06) :1389-1399

[6]

Genetic variation in selenoprotein S influences inflammatory response [J].

Curran, JE ;

Jowett, JBM ;

Elliott, KS ;

Gao, Y ;

Gluschenko, K ;

Wang, JM ;

Azim, DMA ;

Cai, GW ;

Mahaney, MC ;

Comuzzie, AG ;

Dyer, TD ;

Walder, KR ;

Zimmet, P ;

MacCluer, JW ;

Collier, GR ;

Kissebah, AH ;

Blangero, J .

NATURE GENETICS, 2005, 37 (11) :1234-1241

[7]

Activation of the selenoprotein SEPS 1 gene expression by pro-inflammatory cytokines in HepG2 cells [J].

Gao, Y ;

Hannan, NRF ;

Wanyonyi, S ;

Konstantopolous, N ;

Pagnon, J ;

Feng, HC ;

Jowett, JBM ;

Kim, KH ;

Walder, K ;

Collier, GR .

CYTOKINE, 2006, 33 (05) :246-251

[8]

Regulation of the selenoprotein SelS by glucose deprivation and endoplasmic reticulum stress - SelS is a novel glucose-regulated protein [J].

Gao, Y ;

Feng, HC ;

Walder, K ;

Bolton, K ;

Sunderland, T ;

Bishara, N ;

Quick, M ;

Kantham, L ;

Collier, GR .

FEBS LETTERS, 2004, 563 (1-3) :185-190

[9]

Ischemia-induced programmed cell death in astrocytes [J].

Giffard, RG ;

Swanson, RA .

GLIA, 2005, 50 (04) :299-306

[10]

The selenoproteome exhibits widely varying, tissue-specific dependence on selenoprotein P for selenium supply [J].

Hoffmann, Peter R. ;

Hoge, Simone C. ;

Li, Ping-An ;

Hoffmann, Fukun W. ;

Hashimoto, Ann C. ;

Berry, Marla J. .

NUCLEIC ACIDS RESEARCH, 2007, 35 (12) :3963-3973